| Code |
11866
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| Year |
3
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| Semester |
S2
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| ECTS Credits |
6
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| Workload |
PL(15H)/T(30H)/TP(15H)
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| Scientific area |
Biotecnologia
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Entry requirements |
Not-Applicable
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Mode of delivery |
Face-to-face
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Work placements |
Not applicable
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Learning outcomes |
This course unit (CU) is organized into three distinct modules. The goal is to provide to students theoretical and practical knowledge in the field of genetic engineering, namely to understand the various tools of molecular biology that can be used in biotechnology and biochemistry.
At the end of this CU students must be able to:
- Identify the advantages and disadvantages of different types of vectors, as well as the use of prokaryotic and eukaryotic systems.
- Describe the fundamentals of the various molecular tools required for the manipulation of genes and vectors.
- Describe several applications of genetic engineering in the fields of Biotechnology and Biochemistry.
- Use databases and software for acquisition and analysis of DNA sequences.
- Use the PCR technique to amplify a gene.
- Clone a gene into a commercial plasmid, as well as selecting and analysis of recombinant clones.
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Syllabus |
Theoretical Program:
1. Introduction to genetic engineering.
2. Enzymes: restriction enzymes; DNA modifying; DNA ligase.
3. Host cells and vectors: Prokaryotic vs. Eukaryotic.
4. Getting DNA into cells: transformation; transfection; packaging phage DNA in vitro; electroporation; microinjection; biobalistics.
5. Construction of cDNA and genomic libraries.
6. Gene cloning: synthesis of recombinant proteins.
7. The polymerase chain reaction (PCR).
8. Selection, screening, and analysis of recombinants.
9. Bioinformatics in genetics.
10. Applications: Recombinant proteins; genetic modified organisms; gene therapy; Development of novel vectors.
Practical program:
1. To search and analysis of DNA sequences using informatics tools.
2. Gene amplification by PCR.
3. Cloning of PCR product into pGEM-T easy vector, and transformation of competent cells.
4. Plasmid DNA extraction, enzymatic digestion and electrophoresis.
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Main Bibliography |
1. Desmond S.T. Nicholl; An introduction to genetic engineering; 3rd Edition, Cambridge, 2008
2. Arnaldo Videira; Engenharia Genética-Princípios e Aplicações; 2ª Edição, LIDEL, 2011.
3. S.B. Primrose and R.M. Twyman; Principles of gene manipulation and genomics; 7th Edition, Blackwell publishing, 2009.
4. A.J. Nair; Introduction to biotechnology and genetic engineering; Infinity Science Press LLC, 2008.
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Language |
Portuguese. Tutorial support is available in English.
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