Code |
11870
|
Year |
3
|
Semester |
S2
|
ECTS Credits |
6
|
Workload |
PL(15H)/T(30H)/TP(15H)
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Scientific area |
Biotecnologia
|
Entry requirements |
there is not
|
Mode of delivery |
Face to Face with recourse to e-learning.
|
Work placements |
Not applicable.
|
Learning outcomes |
It is intended that students learn the basics about the design, construction, operation and analysis of results obtained with biosensors. Are discussed different types of biosensors in terms of the biological recognition used as well as the methods of transduction. At the end of the course the student should be able to ...
Describe the operating principles and signal transduction in biosensors.
Deciding on the most appropriate biological element, as well as the mode of stopping, for a particular biosensor.
Planning, design, build and evaluate the performance of biosensors.
Perform measurements with biosensors.
Obtain and review the parameters that characterize the operation of a biosensor.
Solve problems related to measurement equipment associated with a biosensor.
Teamwork to solve theoretical and practical problems.
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Syllabus |
Theoretical program
1. Introduction to biosensors. 2. Block diagram of the measurement system. Overview of the more used functional blocks. Most used types of transduction: thermometric; potentiometric; ampermetric; optical; piezoelectric. 3. Components of molecular recognition. Bioaffinity and Biocatalysis. 4. Methods of Immobilization of biological components. Adsorption, encapsulation, Ligação covalente, Cross-linking, Semipermeable membranes. Advantages and disadvantages of using different biological components in biosensors. 5. DNA and SPR biosensors. 6. Study of electronic systems in the functioning of biosensors. 7. Operating parameters and evaluation of biosensors. Practical program
Work 1: Experimental analysis of the functioning of a potentiostat. Work 2: Construction and testing of a reference electrode Work 3: Construction and testing of an ion-selective electrode (ISE) Work 4: Construction and characterization of a phenol biosensor.
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Main Bibliography |
1. Required reading
Electrochemical sensors, biosensors and their biomedical applications / edited by Xueji Zhang, Huangxian Ju, Joseph Wang. Elsevier/Academic Press, 2008.
Bioelectrochemistry : fundamentals, experimental techniques and applications / edited by P.N. Bartlett. Chichester : John Wiley & Sons, 2008.
www.journals.elsevier.com/biosensors-and-bioelectronics/
2.Recommended
Biosensors : fundamentals and applications / edited by Anthony P. F. Turner, Isao Karube and George S. Wilson Oxford : Oxford University, 1987
Teatcher's notes from de e-conteudos platform.
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Language |
Portuguese. Tutorial support is available in English.
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