Code |
12813
|
Year |
2
|
Semester |
S1
|
ECTS Credits |
6
|
Workload |
PL(15H)/T(30H)/TP(15H)
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Scientific area |
Biochemistry
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Entry requirements |
Not applicable.
|
Mode of delivery |
Face to face lectures.
|
Work placements |
Not applicable
|
Learning outcomes |
This Course aims to provide students with a set of knowledge in the area of basic biochemistry that enable them to understand the molecular logic of life. At the end of this course the student should be able to: -Identify the general principles and terminology of Biochemistry; -Recognize and describe the structure and properties of biomolecules; -Explain the relation between the structure and properties of biomolecules and their biological function; -Identify and describe the basic principles of enzymology and enzyme mechanisms; -Apply the acquired knowledge to interpret and to solve problems in the field of Biochemistry; -Perform experimental techniques in the field of Biochemistry and analyze and interpret the results accurately and critically
|
Syllabus |
1 INTRODUCTION TO BIOCHEMISTRY. Origin of biomolecules. The water molecule (properties and buffer solutions). 2 PROTEINS. Structure and properties of amino acids. Stereochemistry and acid-base properties. Hierarchy and structural classification of proteins. Structure and function of proteins. Allosteric proteins: hemoglobin. Purification and characterization of proteins. Properties of enzymes. 3-CARBOHYDRATES. Structure and general properties. Stereoisomerism, mutarotation and anomeric forms. 4. LIPIDS. Classification, structure and properties. 5. NUCLEIC ACIDS. Structure, properties and function. Plasmids. 6. VITAMINS AND COENZYMES. General features of water-soluble and fat soluble vitamins. Coenzyme forms.
EXPERIMENTAL Preparation and testing of biological buffer solutions Determination of protein concentration by Bradford method Study of the kinetics of sucrose hydrolysis by the enzyme invertase
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Main Bibliography |
Required Reading - D.L. Nelson e M.M. Cox (2017) Lehninger, Principles of Biochemistry, 7 ed., Worth Publishers. - A.Quintas, A.P Freire, M.J. Halpern, (2008) Bioquímica - Organização Molecular da Vida, Ed. Lidel - D. Voet, J.G. Voet (2010). Biochemistry. 4ª ed.,New York: J. Wiley & Sons. - J.M. Berg, J.L. Tymoczko, L. Stryer (2011) Biochemistry. 7ª ed., New York: Freeman
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Teaching Methodologies and Assessment Criteria |
Theoretical and theoretical-practical classes of a dynamic nature. Practical classes involve carrying out experimental work relating to theoretical concepts.
A. Teaching Learning Classification (CEA):
Final grade (NF) = 70% Theoretical final grade (NFT) + 30% Practical grade (NP)
-The minimum CEA to pass the UC: 9.5 values.
-This final grade formula applies to frequencies and exams.
-The exam grade is considered a theoretical grade.
-The minimum CEA for admission to the exam: Approval of the practical component (minimum grade of 9.5).
A1-A Theoretical Assessment (70% of NF): completion of 3 tests with a minimum average final grade of 9.5 to pass this component: 1st test 30%+ 2nd test 35%+3rd test 35%
A.2-A Practical Assessment 30% of NF = 50% x MT + 50% x TTP
MT – Average of the 2 mini-tests TTP – Score on the theoretical-practical test or theoretical-practical recovery test.
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Language |
Portuguese. Tutorial support is available in English.
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