Biochemistry I

Code	13645
Year	2
Semester	S1
ECTS Credits	6
Workload	PL(15H)/T(30H)/TP(15H)
Scientific area	Ciências Biológicas e Biomédicas
Entry requirements	Not applicable.
Mode of delivery	Face to face lectures.
Work placements	Not applicable
Learning outcomes	This Course aims to provide students with a set of knowledge in the area of basic biochemistry that enable them to understand the molecular logic of life. At the end of this course the student should be able to: -Identify the general principles and terminology of Biochemistry; -Recognize and describe the structure and properties of biomolecules; -Explain the relation between the structure and properties of biomolecules and their biological function; -Identify and describe the basic principles of enzymology and enzyme mechanisms; -Apply the acquired knowledge to interpret and to solve problems in the field of Biochemistry; -Perform experimental techniques in the field of Biochemistry and analyze and interpret the results accurately and critically
Syllabus	1 INTRODUCTION TO BIOCHEMISTRY. Origin of biomolecules. The water molecule (properties and buffer solutions). 2 PROTEINS. Structure and properties of amino acids. Stereochemistry and acid-base properties. Hierarchy and structural classification of proteins. Structure and function of proteins. Allosteric proteins: hemoglobin. Purification and characterization of proteins. Properties of enzymes. 3-CARBOHYDRATES. Structure and general properties. Stereoisomerism, mutarotation and anomeric forms. 4. LIPIDS. Classification, structure and properties. 5. NUCLEIC ACIDS. Structure, properties and function. Plasmids. 6. VITAMINS AND COENZYMES. General features of water-soluble and fat soluble vitamins. Structure and biological role of vitamins. Coenzymes. 7.EXPERIMENTAL Preparation and testing of buffer solutions. Potentiometric titration of amino acids. Determination of protein concentration by Bradford method. Study of the kinetics of sucrose hydrolysis by the enzyme invertase.
Main Bibliography	1- D.L. Nelson e M.M. Cox (2017). Lehninger Principles of Biochemistry: International Edition, 7th ed., W.H.Freeman & Co Ltd, NY, USA, ISBN 1464126119 2- J.M. Berg, J.L. Tymoczko , L. Stryer (2015). Biochemistry. 8th ed., Freeman, New York, USA, ISBN 1464126100 3- A. Quintas, A.P Freire, M.J. Halpern (2008). Bioquímica Organização Molecular da Vida, Ed. Lidel, Lisboa, Portugal, ISBN 9789727574315 4-D. Voet, J.G. Voet (2011). Biochemistry. 4th ed., John Wiley & Sons, New York, USA, ISBN 0121822117
Teaching Methodologies and Assessment Criteria	Theoretical and theoretical-practical classes of a dynamic nature. Practical classes involve carrying out experimental work relating to theoretical concepts. A. Teaching Learning Classification (CEA): Final grade (NF) = 70% Theoretical final grade (NFT) + 30% Practical grade (NP) -The minimum CEA to pass the UC: 9.5 values. -This final grade formula applies to frequencies and exams. -The exam grade is considered a theoretical grade. -The minimum CEA for admission to the exam: Approval of the practical component (minimum grade of 9.5). A1-A Theoretical Assessment (70% of NF): completion of 3 tests with a minimum average final grade of 9.5 to pass this component: 1st test 30%+ 2nd test 35%+3rd test 35% A.2-A Practical Assessment 30% of NF = 50% x MT + 50% x TTP MT – Average of the 2 mini-tests TTP – Score on the theoretical-practical test or theoretical-practical recovery test.
Language	Portuguese. Tutorial support is available in English.