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Laboratory/Clinical Rotations II

Laboratory/Clinical Rotations II

Code	11017
Year	1
Semester	S2
ECTS Credits	5
Workload	TP(40H)
Scientific area	Ciências Farmacêuticas/Bioquímica/Medicina/Biomedicina
Entry requirements	Without entry requirements.
Mode of delivery	Face to face
Learning outcomes	a) To know the fundamentals of different practical fundamentals of laboratory or clinical techniques; b) To have the first contact with the chosen techniques in a research laboratory performing real experiences; c) To know how to perform the techniques that are necessary to develop the PhD research project. At the end of the course the student should be able to: a) To explain the theoretical/practical fundamentals of laboratory or clinical techniques; b) To use the knowledge and skills acquired to perform scientific experiments with the learned techniques.
Syllabus	r01: Western Blot (2 points, 12h) r02: Pulsed field gel electrophoresis (2 points, 16h) r03 - Immunohistochemistry (1 point, 8h) r04: Immunocytochemistry (1 point, 8h) r05: Fluorescence microscopy (1 point, 8h) r06: Intracellular calcium imaging (1 point, 8h) r07: Stereotactic surgery (1 point, 8h) r08: Analysis of gene expression by real-time PCR (1 point, 8h) r09: Primary cultures (1 point, 8h) r10: Cell line culture (1 point, 8h) r11. Flow cytometry (1 point, 8h) r12: Apoptosis assay (1 point, 8h) r13: HPLC (1 point, 8h) r14: Biosensor (2 points, 16h) r15: Administration of drugs to animals (1 point, 8h) r16: Transport Studies using Ussing Chambers (2 points, 16h) r17: In vitro metabolism studies (2 points, 16h) r18: Determination of the structure of molecules by NMR (2 points, 16h) r19: Extraction and purification of nucleic acids (2 points, 16h) r20: Structural evaluation of biomolecules by circular dichroism (1 point, 8h)
Main Bibliography	Bonner P. Basic Bioscience Laboratory Techniques. Willey-Blackwell 2011; Estridge BH. Basic Clinical Laboratory Techniques. Cengage Learning 2011; Longobardi Givan A. Flow Cytometry First Principles 1992; Neher E. The use of the patch clamp technique to study second messenger- mediated cellular events. Neurosci 1998; Snyder LR et al. Introduction to Modern Liquid Chromatography. John Wiley & Sons; Bernard RE. The Regulation of Animal Research and the Emergence of Animal Ethics. Theor. Med. Bioeth 2006; Regine W et al. Principles of Stereotactic Surgery. Springer 2008; Schwartz DC et al.. Separation of yeast chromosome-sized DNAs by pulsed field gradient gel electrophoresis. Cell 1984; Silverstein RM et al. Spectrometric Identification of Organic Compounds. Wiley 1991; Moller LJ et al. Overview of Biacore Systems and Their Applications. Curr Protoc Protein Sci 2006; http://www.imperial.ac.uk/people/j.nicholson http://www.nature.com/nprot/journal/v5/n6/abs/nprot.2010.45.html
Language	Portuguese. Tutorial support is available in English.