| Code |
11863
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| Year |
3
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| Semester |
S1
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| ECTS Credits |
6
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| Workload |
PL(15H)/T(30H)/TP(15H)
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| Scientific area |
Biotecnologia
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Entry requirements |
Students should have basic knowledge of physical-chemistry and calculus.
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Mode of delivery |
Presential
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Work placements |
They are not antecipated.
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Learning outcomes |
Students should acquire the necessary and sufficient knowledge to implement and optimize processes and unit operations common in Biochemical Engineering, in particular, in cell disintegration, transport phenomena in bioseparation and purification of products obtained in bioreactors.
After this Curricular Unit the student should be able to implement, intervene and examine strategies for cell rupture, separation and purification of extracellular and intracellular products and extraction of biological products. Consequently, they should be able to describe and discuss the main parameters influencing the performance of unit operations used for solid-liquid separation (filtration, Sedimentation, Flotation, Centrifugation and membrane Technologies) and purification of soluble products (liquid-liquid Extraction, Adsorption, Precipitation, Electrophoresis and Chromatography).
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Syllabus |
- Context and importance of unit operations of separation in biotechnology.
- “Downstream Processing" (DSP) implementation strategies, objectives and examples.
- Cell Disintegration: Fundamentals of mechanical and non-mechanical methods (advantages and disadvantages). Examples and application contexts.
- Processes and unit operations for solid-liquid separations : Foundations and contexts of implementation. Sedimentation. Flotation. Centrifugation. Conventional filtration. Membrane Technologies.
- Physicochemical Processes of separation and purification: Foundations and contexts of implementation. Precipitation. Liquid -Liquid Extraction (traditional, aqueous two-phase, reverse micelles). Adsorption of biomolecules. Chromatography (Process. Modes of operation. Performance factors. Separation performance metrics. Chromatographic support.) Electrophoresis of proteins.
- Analysis of results from sequential application of the separation and purification unit operations.
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Main Bibliography |
Main titles
Bailey, J.E. and Ollis, D.F. (1987). Biochemical Engineering Fundamentals. McGraw-Hill, New York, USA.
H.C. Vogel, H.C. and Todaro, C.L. (1997). Fermentation and Biochemical Engineering Handbok: Principles, Process design and Equipment. Noyes Publications, USA.
Mulder, M. (1998). Basic Principles of Membrane Technology. Kluwer Academic Publishers, Nederland
Complementary Titles
Cooke, M. and Poole, C.F. (1996). Encyclopaedia of Separation Science. Academic Press, USA.
Cazes, J. (2004). Encyclopaedia of Chromatography. Marcel Dekker.
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Language |
Portuguese. Tutorial support is available in English.
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