| Code |
8463
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| Year |
1
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| Semester |
S2
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| ECTS Credits |
6
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| Workload |
PL(30H)/T(30H)
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| Scientific area |
Industrial Chemistry
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Entry requirements |
Not applicable
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Mode of delivery |
Face to face with recourse to e-learning.
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Work placements |
Not applicable.
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Learning outcomes |
It is intended that students learn the basics about the design, construction, operation and analysis of results obtained with biosensors. Are discussed different types of biosensors in terms of the biological recognition used as well as the methods of transduction.
At the end of the course the student should be able to:
1. Describe the operating principles and signal transduction in biosensors.
2. Deciding on the most appropriate biological element, as well as the mode of stopping, for a particular biosensor.
3. Planning, design, build and evaluate the performance of biosensors.
4. Perform measurements with biosensors.
5. Obtain and review the parameters that characterize the operation of a biosensor.
6. Solve problems related to measurement equipment associated with a biosensor.
7. Carry out teamwork to solve theoretical and practical problems.
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Syllabus |
Theoretical program
1. Introduction to biosensors.
2. Block diagram of the measurement system. Overview of functional blocks.
2.1 Types of energy transduction most frequently used: thermometric; Potentiometric, Amperimetric, Optical, piezoelectric.
3. Components of molecular recognition.
3.1 Biocatalysis and bioaffinity.
4. Methods for immobilization of biological components.
4.1 Adsorption.
4.2 Encapsulation.
4.3 Covalent.
4.4 Cross-linking.
4.5 Semi-permeable membranes.
5. Advantages and disadvantages of using different biological components in biosensors.
6. Biosensors DNA and biosensors SPR.
7. Operation of Biosensors.
Practical program
Work 1: Construction and testing of a reference electrode
Work 2: Construction and characterization of a biosensor for glucose.
Work 3: Construction and characterization of a biosensor for paracetamol.
Work 4: Experimental analysis of the operation of a potentiostat.
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Main Bibliography |
1. Required reading
Electrochemical sensors, biosensors and their biomedical applications / edited by Xueji Zhang, Huangxian Ju, Joseph Wang. Elsevier/Academic Press, 2008.
Bioelectrochemistry : fundamentals, experimental techniques and applications / edited by P.N. Bartlett. Chichester : John Wiley & Sons, 2008.
www.journals.elsevier.com/biosensors-and-bioelectronics/
2.Recommended
Biosensors : fundamentals and applications / edited by Anthony P. F. Turner, Isao Karube and George S. Wilson Oxford : Oxford University, 1987
Teatcher's notes from de e-conteudos platform.
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Language |
Portuguese. Tutorial support is available in English.
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