| Code |
13705
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| Year |
5
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| Semester |
S1
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| ECTS Credits |
3
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| Workload |
PL(10H)/T(12H)/TP(8H)
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| Scientific area |
Ciências Farmacêuticas
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Entry requirements |
Prior knowledge of toxicology, clinical analysis and analytical methods
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Learning outcomes |
Provide the students with an integrated view of the basic principles of laboratory equipment, instrumental techniques and the analytical methodologies most commonly used in the field of bioanalytical applications in the laboratories of toxicology and clinical analysis. The ultimate objective is for students to gain a deeper understanding of the technical/technological aspects of all laboratory activities, namely developing skills for an adequate and efficient use of laboratory equipment, with a good knowledge of the basic operating principles, and for the efficient optimization, implementation, validation and quality control of analytical methods. This UC will provide the basis for the development of a future professional activity in a clinical laboratory in the fields of toxicology and clinical analysis, contemplating the advances that have occurred in the last years in both instrumental and molecular techniques.
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Syllabus |
Introduction - The Laboratory of Toxicology and Clinical Analyses 1 - Laboratory terminology. Analytical methodologies: Development and validation of methods of analysis and basic applied statistics. Quality control. Legislation concerning manufacture and placing on the market. EC declaration of conformity. 2 - Instrumental techniques - Basic principles of operation and examples of use: Techniques. 3 - Automation. Historical perspective and technological developments. General concepts about automation. 4- 4. Point of care testing. Practical component. The practical component of the curricular unit will consist of laboratory work to highlight the basic principles of instrumentation operation and to carry out procedures for concepts’ application, namely those related to the development and validation of analytical methods: detection of drugs of abuse and drugs by immunoassays (ELISA) and determination of drugs of abuse by gas chromatography - mass spectrometry.
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Main Bibliography |
Guidelines to Clinical and Laboratory Standards Institute. Guideline on bioanalytical method validation- European Medicines Agency. Guideline on bioanalytical method validation- Food and Drug Administration. Guidelines to Scientific Working Group for Forensic Toxicology. Gerald J. Kost (Ed). ; Handbook of Clinical Automation, Robotics, and Optimization. , Wiley-Interscience, 1996. Michael G. Bissell and John R. Petersen; Automated Integration of Clinical Laboratories: A Reference, AACC, 1998. J.M. González de Buitrago. Técnicas y Métodos de Laboratorio Clínico. 3ª Edición. Elsevier España, 2010. Robert J. Flanagan et al. Fundamentals of Analytical Toxicology: Clinical and Forensic, 2nd Edition. John Wiley & Sons, 2020. Rosado T et al. The Role of liquid Chromatography in toxicological Analysis. In: High- performance liquid Chromatography: types, parameters and applications. Nova Sciences Publishers, 2018. B. Estridge (Ed.) Basic Clinical Laboratory Techniques 6th Edition. 2021.
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Teaching Methodologies and Assessment Criteria |
Assessment criteria:
Classification of Teaching Learning:: a group work (50%), laboratory performance (20%) and a written test on theoretical content (30%)
The laboratory performance is evaluated by the student's performance during the practical works.
Final mark of 9.5 or more.
Classification of Exam: a group work (50%), laboratory performance (20%) and a written test on theoretical content (30%)
Final mark of 9.5 or more.
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Language |
Portuguese. Tutorial support is available in English.
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