| Code |
15044
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| Year |
1
|
| Semester |
S2
|
| ECTS Credits |
5
|
| Workload |
TP(40H)
|
| Scientific area |
Biomedicina/Medicina/Bioquímica
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|
Entry requirements |
Without entry requirements
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|
Learning outcomes |
Upon completion of the course, the student should:
- Have an overview of several basic and advanced techniques of fluorescence microscopy used in biomedical research.
- Have acquired fundamental notions on image analysis, such as digitizing, image enhancement, segmentation, and feature classification.
- Know how to select the image acquisition mode suitable for the intended purpose.
- Perform manual and automatic measurements using image analysis tools.
- Perform object tracking.
-Understand the applications and limitations of each technique.
- Know and apply the fundamentals of ethics in Image analysis.
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Syllabus |
- Basic concepts in fluorescence microscopy: different fluorochromes, excitation methods, emission characteristics, purpose, and uses.
- Basic concepts about digital images.
- Basic methods of computerized image analysis
- Multidimensional data (2D, 3D, time-lapse, position)
- Image processing in preparation for analysis, image enhancement
- Object/particle analysis, intensity, and morphometric measurements
- Tracking motion of cells or particles
- Extraction of quantitative data
- Automatic analysis of large data sets
- The course will also address ethical issues associated with the proper acquisition and analysis of images.
- The course also introduces various freely available software tools (ImageJ/Fiji) and some commercial software (Imaris).
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Main Bibliography |
Fluorescence Microscopy: From Principles to Biological Applications, 2nd Edition (2017)
Ulrich Kubitscheck (Editor) ISBN: 978-3-527-33837-5 Wiley-Blackwell
Bioimage Data Analysis, Edited by Kota Miura, Wiley-VCH
ePDF ISBN: 978-3-527-80092-6
Quantitative Imaging in Cell Biology, Methods in Cell Biology, Volume 123 (2014)
Editors Jennifer C. Waters & Torsten Wittmann
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Language |
Portuguese. Tutorial support is available in English.
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