| Code |
15047
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| Year |
1
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| Semester |
S2
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| ECTS Credits |
5
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| Workload |
TP(40H)
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| Scientific area |
Biomedicina/Medicina/Bioquímica
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Entry requirements |
Without requirements
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Learning outcomes |
The aim of the advanced course is to introduce to doctoral students two immunological techniques widely used in biomedical research, advanced flow cytometry (FCM) and immunoprecipitation of proteins (IPP). Importantly, the advanced course allows the students to get acquainted with the diversity of applications of these two techniques, both at basic and clinical level. At the end of the course the students should be able to:
— To understand and describe the basics of these two techniques.
— To know how to proceed experimentally to use them.
— To be able to evaluate the suitability and limitations of the techniques for research studies.
— To interpret the results obtained during the laboratorial classes and those in scientific reports.
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Syllabus |
THEORETICAL CLASSES
- Basic concepts of Flow Cytometry (FCM) and applications
- Basic concepts of Immunoprecipitation of Proteins (IPP) and applications
THEORETICAL AND PRACTICAL CLASSES
- Presentation and discussion of clinical cases applied to FCM and IPP.
- Presentation and discussion of selected original papers.
- Analysis and discussion of the results of the Hans-on Lab.
LABORATORY CLASSES
- Isolation of peripheral blood mononuclear cells (PBMC) from buffy-coats.
- Culture of PBMC in the absence and presence of mitogenic stimuli.
- Characterization of the phenotype of freshly collected and activated PBMC by FCM.
- Characterization of cell surface receptors in freshly collected and activated PBMC by IPP.
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Main Bibliography |
- A Beginner’s Guide to Flow Cytometry. (2014) Innova Biosciences.
- Adan A, Alizada G, Kiraz Y, Baran Y, Nalbant A. (2017) Flow cytometry: basic principles and applications. Crit Rev Biotechnol. 37(2):163-176.
- McKinnon KM. (2018). Flow cytometry: An overview. Curr Protoc Immunol. 120:5.1.1–5.1.11.
- Brummelman J, PilIow K, Lugli E. (2018) The Single-Cell Phenotypic Identity of Human CD8+ and CD4+ T Cells. International Review of Cell and Molecular Biology, 341:1-62.
- Carneiro DG, Clarke T, Davies CC, Bailey D. (2015) Identifying novel protein interactions: Proteomic methods, optimisation approaches and data analysis pipelines. Methods 95:46–54.
- Cozzolino F, Iacobucci I, Monaco V, Monti M. (2021) Protein-DNA/RNA Interactions: An Overview of Investigation Methods in the -Omics Era. J. Proteome Res. 20:3018-3030.
- Immunoprecipitation: Overview and Technical TIPPs. (2019) Proteintech.
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Teaching Methodologies and Assessment Criteria |
TEACHING METHODOLOGIES
- Interactive lectures and webinars by local and, eventually, invited speakers.
- A heavy load Hands-on Lab component using FCM and IPP.
- Presentation and discussion of original research papers.
- Presentation and discussion of the results of the Hands-on Lab.
EVALUATION CRITERIA
- Continuous assessment (100%).
OTHER
- Analysis SWOT of the Advanced Course.
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Language |
Portuguese. Tutorial support is available in English.
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