| Code |
15871
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| Year |
3
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| Semester |
S2
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| ECTS Credits |
6
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| Workload |
PL(15H)/T(30H)/TP(15H)
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| Scientific area |
Biotecnologia
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Entry requirements |
Students should have basic knowledge of Physical-Chemistry and Calculus.
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Learning outcomes |
Students should acquire the necessary and sufficient knowledge to implement and optimize processes and unit operations common in
Biochemical Engineering, in particular, in cell disintegration, transport phenomena in bioseparation, recovery and separation/purification of
products obtained in bioreactors.
After this Curricular Unit the student should be able to implement, examine and manipulate strategies for cell rupture, recovery, separation and purification of biological products obtained both extracellularly and intracellularly. Consequently, they should be able to describe and discuss the main parameters influencing the performance of unit operations and analytical methods used for solid-liquid separation (filtration, Sedimentation, Flotation, Centrifugation and membrane Technologies) and separation/purification of soluble products (liquid-liquid Extraction, Adsorption, Precipitation, Chromatography Electrophoresis and Lyophilization).
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Syllabus |
- Context and importance of biotechnology separation unit operations.
- “Downstream Processing”: Implementation strategies, objectives and examples.
- Cell disintegration: Fundamentals of mechanical and non-mechanical methods (advantages/disadvantages). Examples and application contexts.
- Processes and unit operations for solid-liquid separations: Sedimentation. Flotation. Centrifugation. Conventional filtration. Membrane Technologies.
- Physical-chemical processes of separation and purification: Precipitation. Adsorption of biomolecules. Chromatography. Liquid-Liquid Extraction.
- Protein electrophoresis. Lyophilization.
- Analysis of results of sequential application of unit operations in recovery and separation/purification.
Lab works:
* Recovery and Precipitation of Soluble COMT Isoform.
* Purification by Chromatography of the COMT Soluble Isoform.
* Liquid - liquid extraction with aqueous two-phase systems.
* SDS-PAGE electrophoresis.
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Main Bibliography |
- Carta, G. and Jungbauer, A. (2020) Protein Chromatography. Process Development and Scale-
Up, 2nd Edition, Wiley-VCH: Weinheim, Germany.
- Ahuja S. (ed.) (2000). Handbook of Bioseparations. Academic Press, San Diego, USA.
- Mulder, M. (1998). Basic Principles of Membrane Technology. Kluwer Academic Publishers, Nederland.
- H.C. Vogel, H.C. and Todaro, C.L. (1997). Fermentation and Biochemical Engineering Handbok: Principles, Process design and Equipment. Noyes Publications, USA.
- Cooke, M. and Poole, C.F. (1996). Encyclopaedia of Separation Science. Academic Press, USA.- Kennedy, J.F., Cabral, J. M.S. (1993). Recovery processes for biological materials, Wiley.
- Kennedy, J.F., Cabral, J.M.S. (eds.) (1993). Recovery Processes for Biological Materials, John Wiley & Sons, Chichester, UK.
- Bailey, J.E. and Ollis, D.F. (1986). Biochemical Engineering Fundamentals. McGraw-Hill, New York, USA.
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Teaching Methodologies and Assessment Criteria |
To achieve course desired learning outcomes and competencies, lectures, theoretical-practical classes and laboratory practices are taught. In theoretical classes the teacher presents the topics and student’s participation is required and stimulated for the analysis and discussion of several examples. When solving topic problems, it is required knowledge application of unit operations and processes fundamentals. Experimental activities in practical classes are held in groups in order to recover and purify intracellular products, involving the application of the topics taught. In these classes is provided a protocol and groups are required to prepare a report (that includes presentation of results, analysis, discussion and conclusions).
The evaluation includes two tests, with questions addressed to the fundamentals and practical issues. The experimental classes are evaluated in group including the performance during classes and the reports presented.
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Language |
Portuguese. Tutorial support is available in English.
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