Biossensores

Imagem d@ Cristina Dias-Cabral [Ficheiro Local]
Cristina Dias-Cabral
Código:
15874
Ano:
3
Semestre:
S2
Créditos ECTS:
6
Carga Horária:
PL(15H)/T(30H)/TP(15H)
Área Científica:
Biotecnologia
Learning outcomes:
The learning objectives aim for students to acquire solid knowledge of biosensors, including their architecture, molecular recognition components, transducers, and analytical validation, as well as to understand their practical applications. The skills developed include critical analysis of measurement systems, selection of immobilization methods, interpretation of performance parameters, and evaluation of innovative solutions. Competencies encompass teamwork, scientific communication, and autonomy in problem-solving. The teaching method, combining theoretical lectures, case discussions, study of recent applications, and development of mini-projects, ensures alignment with these objectives, enabling students to integrate theory and practice, develop experimental and analytical skills, and apply knowledge to real and innovative contexts.
Syllabus:
1. Overview of Biosensors:
1.1. Introduction to biosensor architecture.
1.2. Block diagram of the measurement system.
1.3. Ideal requirements.
1.4. Classification of biosensors.
2. Molecular Recognition Components:
2.1. Different types of molecular recognition components – General aspects, advantages, and disadvantages.
2.2. Methods for immobilizing molecular recognition components.
3. Transducers Used in Biosensors:
3.1. Electrochemical transducers. 3.2. Optical transducers.
3.3. Miniaturization of biosensors – application of nanomaterials.
3.4. Acoustic wave transducers.
3.5. Thermal transducers.
4. Performance Parameters and Evaluation of Biosensors:
4.1. Validation requirements for biosensors.
4.2. Analytical validation characteristics of biosensors.
5. Recent Applications of Biosensors:
5.1. Health (Point-of-care sensing).
5.2. Food safety and water monitoring.
5.3. Environmental pollutants.
6. Mini-projects.
Teaching Methodologies and Assessment Criteria:
1. Continuous Assessment
- Assessment 1 – 20%
- Assessment 2 – 20%
- Badges – 15% (the 4 best selected from chapters 2; 3.1; 3.2; 3.3 to 3.5; 4 are evaluated)
- Presentation on recent biosensor applications in one of the areas: Health, Food Safety, Water Monitoring, and Environmental Pollutants – 10%
- Mini-projects – 35% (Project-Based Learning – average of the grades obtained in the 4 mini-projects carried out)
2. Exam Assessment
- Exam – 40%
- Badges – 15%
- Presentation on recent biosensor applications – 10%
- Mini-projects – 35% (average of the grades obtained in the 4 mini-projects carried out)
3. Attendance Requirement
- 9.5/20 average in mini-projects (MP).
4. Passing Criteria
- 9.5/20 average in the MP + 9.5/20 average in continuous assessments
or
- 9.5/20 average in the MP + 9.5/20 in the exam.
Main Bibliography:
• Biosensors: Fundamentals, Emerging Technologies, and Applications / edited by Edited by Sibel A. Ozkan, Bengi Uslu, and Mustafa Kemal Sezgintürk, CRC Press, Taylor & Francis Group, 2023.
• Biosensors : fundamentals and applications / edited by Bansi Dhar Malhotra and Chandra Mouli Pandey, Smithers Rapra Technology Ltd, 2017
• Chemical Sensors and Biosensors: fundamentals and Applications / edited by Florinel-Gabriel Banica, John Wiley & Sons, 2012.
• www.journals.elsevier.com/biosensors-and-bioelectronics/
• Miscellaneous articles.
• Teachers notes on the Moodle platform.
Language:
Portuguese. Tutorial support is available in English.