Applied Microbiology

Code	12514
Year	3
Semester	S1
ECTS Credits	6
Workload	PL(30H)/TP(30H)
Scientific area	Biomedical Sciences
Entry requirements	Not applicable
Learning outcomes	At the end of the course unit the student should be able to: - Recognize the importance of microbiology in the context of practical life, the healthy environment - Understand the importance and functions of proteins, vitamins and minerals. - State the basic structural characteristics of various microorganisms (bacteria, fungi, parasites and viruses) and establish their relationship with humans as etiological agents of infection. - Describe mechanisms of action of some antibiotics and explain microbial resistance - Understand the immune system and its way of acting. The defences of our body. - Correctly perform the aseptic technique for handling microorganisms. - Use typical laboratory methodologies for the study of microbiology: morphological characterization, metabolic study and susceptibility to antimicrobials.
Syllabus	- Introduction to Microbiology: history, objective and importance. Distinction between prokaryotic and eukaryotic cellular organization. Taxonomy microbial. Microorganisms as disease-causing agents. - Importance of microorganisms. - Differences between main groups of microorganisms: bacteria, fungi, parasites and viruses. - Bacteria: cellular organization and ultrastructure. Bacterial nutrition. Bacterial metabolism. Bacterial genetics. - Antibiotherapy and mode of action of some antibiotics. Control of microorganisms by physical and chemical agents. Mechanism of antibiotic resistance. - Notions of immunology: nonspecific and specific host defenses. Practical applications of immunology. - Safety rules in the Microbiology laboratory. Aseptic technique. Culture media in - Microbiology and its use in the laboratory. Viable bacteria count on plate. Isolation of bacterial cultures and identification.
Main Bibliography	- Willey, J., Sherwood, L.M.; Wo -Hill Higher Education, New York, NY. Eighth Edition - Madigan, M. T., Martinko, J.M., Parker, J. (2008). Brock Biology of Microorganisms, Prentice Hall Editions, 12th edition - Canas Ferreira, W.F., Sousa J. C., Lima, N. (2010) Microbiologia. Lidel, Edições técnicas - Cappucino J. G., Sherman, N. ( 2008 ) Microbiology - a laboratory Manual, Addison-Wesley Publishing Company, 8a ed. - Harley, J. (2010) Microbiology Lab Manual, McGrawHill - Tortora, J. Gerard, Funke, Berdell R., Case, Christine L.,(2008) Microbiologia, Artmed, 8ª Edição
Teaching Methodologies and Assessment Criteria	The methodology used in this course is based on the definition of the objectives to be achieved, attitudes and skills to be developed, and the skills to be acquired. Student-centred learning is expository in class, as well as active training methodologies, with a practical component in the laboratory context. Based on the organized and available learning materials, time zones of intra-group self-learning are delimited with reporting and sharing of this acquisition of skills by students expositively in the classroom context. The evaluation will be made through a written test, as well as through research work, synthesis and oral presentation according to the syllabus taught in the course.
Language	Portuguese. Tutorial support is available in English.