Applied Microbiology

Code	12514
Year	3
Semester	S1
ECTS Credits	6
Workload	PL(30H)/TP(30H)
Scientific area	Biomedical Sciences
Entry requirements	Not applicable
Learning outcomes	At the end of the course unit the student should be able to: - Recognize the importance of microbiology in the context of practical life, the healthy environment - Understand the importance and functions of proteins, vitamins and minerals. - State the basic structural characteristics of various microorganisms (bacteria, fungi, parasites and viruses) and establish their relationship with humans as etiological agents of infection. - Describe mechanisms of action of some antibiotics and explain microbial resistance - Understand the immune system and its way of acting. The defences of our body. - Correctly perform the aseptic technique for handling microorganisms. - Use typical laboratory methodologies for the study of microbiology: morphological characterization, metabolic study and susceptibility to antimicrobials.
Syllabus	- Introduction to Microbiology: history, objective and importance. Distinction between prokaryotic and eukaryotic cellular organization. Taxonomy microbial. Microorganisms as disease-causing agents. - Importance of microorganisms. - Differences between main groups of microorganisms: bacteria, fungi, parasites and viruses. - Bacteria: cellular organization and ultrastructure. Bacterial nutrition. Bacterial metabolism. Bacterial genetics. - Antibiotherapy and mode of action of some antibiotics. Control of microorganisms by physical and chemical agents. Mechanism of antibiotic resistance. - Notions of immunology: nonspecific and specific host defenses. Practical applications of immunology. - Safety rules in the Microbiology laboratory. Aseptic technique. Culture media in - Microbiology and its use in the laboratory. Viable bacteria count on plate. Isolation of bacterial cultures and identification.
Main Bibliography	- Willey, J., Sherwood, L.M.; Wo -Hill Higher Education, New York, NY. Eighth Edition - Madigan, M. T., Martinko, J.M., Parker, J. (2008). Brock Biology of Microorganisms, Prentice Hall Editions, 12th edition - Canas Ferreira, W.F., Sousa J. C., Lima, N. (2010) Microbiologia. Lidel, Edições técnicas - Cappucino J. G., Sherman, N. ( 2008 ) Microbiology - a laboratory Manual, Addison-Wesley Publishing Company, 8a ed. - Harley, J. (2010) Microbiology Lab Manual, McGrawHill - Tortora, J. Gerard, Funke, Berdell R., Case, Christine L.,(2008) Microbiologia, Artmed, 8ª Edição
Language	Portuguese. Tutorial support is available in English.