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General Microbiology

Code 12882
Year 1
Semester S2
ECTS Credits 6
Workload PL(30H)/T(30H)
Scientific area Biotecnologia
Entry requirements Not applicable
Mode of delivery Face to face lectures
Work placements Not applicable
Learning outcomes This Course is intended that students learn the fundamentals of microbiology, including structure and biology of microorganisms, microbial diversity and ecology, control of microbial growth as well as the basic techniques used in microbiology. At the end of this UC students should be able to:
-Recognize the importance of microbiology in the context of practical life, environment and health;
-Recognize and describe characteristics of different classes of microorganisms;
-Identify and describe the principles of nutrition and microbial genetics;
-Describe mechanisms of action of physical and chemical methods in the control of microbial growth;
-Perform laboratory procedures used in the study of microorganisms and follow the safety rules in microbiology;
-Work in groups and communicate to others knowledge in microbiology;
-Analyze and interpret experimental results with scientific rigor and critical thinking
Syllabus 1.INTRODUCTION TO MICROBIOLOGY
2.CELLULAR MICROBIOLOGY - Microorganisms prokaryotes and eukaryotes: ultrastructure and cellular function.
3.MICROBIAL METABOLISM- nutritional requirements. Energetic and biosynthetic metabolism.
4.GROWTH AND CONTROL - Growth kinetics and cell death. Batch and continuous culture. Control of growth by physical and chemical methods. Chemotherapeutic agents.
5.MICROBIAL GENETICS – bacterial Genome. Plasmids. Mutagenicity tests. Processes of horizontal gene transfer. Mobile DNA elements
6.MICROBIAL DIVERSITY - classical and molecular taxonomy, evolution, phylogeny and classification. General caracteristics of Bacteria, fungi, parasites and viruses.
7.LABORATORY CLASSES - Safety in the microbiology lab. Preparation and use of culture media. Isolation and culture of viable bacteria count in plate. Techniques for staining microorganisms. Identification of microorganisms. Antimicrobial susceptibility testing. Genetic transformation of microorganisms
Main Bibliography 1. Required reading

• Willey, J., Sherwood, L.M.; Woolverton, C.J. (2011). Prescott’s Microbiology, Eighth Edition, McGraw-Hill Higher Education, New York, NY.
• Canas Ferreira, W.F., Sousa J. C., Lima, N. (2010) Microbiologia. Lidel, Edições técnicas
• Domingues, F., Ferreira, S. Manual Prático de Microbiologia Geral, Universidade da Beira Interior, Covilhã, 2012

2. Recommended reading

• Madigan, M. T., Martinko, J.M., Parker, J. (2008). Brock Biology of Microorganisms, Prentice Hall Editions, 12th edition
• Cappucino J. G., Sherman, N. ( 2008 ) Microbiology- a laboratory Manual, 8a ed., Addison-Wesley Publishing Company.
Teaching Methodologies and Assessment Criteria The student evaluation is carried out continuously, considering three evaluation factors, with the following weighting: (70% T) + (30% P); T-score of two partial tests or final exam, P-evaluation of the results analysis and discussion and written test.
Language Portuguese. Tutorial support is available in English.
Last updated on: 2020-05-14

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