| Code |
13442
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| Year |
1
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| Semester |
S2
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| ECTS Credits |
6
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| Workload |
PL(30H)/T(30H)
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| Scientific area |
Biochemistry
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Entry requirements |
non applicable
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Mode of delivery |
Face-to-Face
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Work placements |
Not aplicable
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Learning outcomes |
1. To acquire general knowledge in microbiology and to know the microorganisms taxonomic position;
2. To know structural differences between microorganisms and their nutritional, growth and reproduction requirements;
3. To know the most important microorganisms related to human diseases and their mechanisms of pathogenicity: bacteria, fungi, virus and parasites;
4. To associate microorganisms to the caused infection and to know its implication in the human organism;
5. To know for each microorganism their characteristics implicated in investigation studies;
6. To recognize the importance of some microorganisms in the biotechnology development;
7. To understand and to perform laboratorial procedures commonly used in diagnosis and investigation microbiology.
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Syllabus |
THEORY:
1. Introduction; Taxonomy; Pathogenicity; vaccines; laboratorial diagnosis;
2. Bacteriology: anatomy, physiology, growth and replication; genetic; biotechnology; microorganisms and infections, preventing and treatment;
3. Parasitology: life cycle; pathogenicity and laboratorial diagnosis; Protozoa and Methazoa;
4.Micology: nutrition, metabolism and reproduction; Mucocutaneous and systemic mycoses;
5. Virology – replication, infections; diagnosis methods; DNA and RNA virus.
LABORATORIAL: Introduction to microbiology laboratory; safety rules; sterilization processes. Optical microscopy. Observation of bacteria and yeast slides. Culture media: types and preparation. Aseptic technique. Microorganisms manipulation and isolation. Slides preparation. Simple and Gram stain method; Ziehl Neelsen (observation); Capsule and spores stain. Bacterial morphology. Microorganisms mobility. Growth curve Biochemical tests. Antibiotic susceptibility determination. PCR.
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Main Bibliography |
- João Carlos F. de Sousa, Wanda Canas Ferreira, Nelson Lima. Microbiologia 2010, Lidel
- Wanda Ferreira e João Carlos F de Sousa. Microbiologia Volumes II e III 2007 Lidel
- João Carlos Sousa, Cristina Abreu, Fátima Cerqueira. Microbiologia: Protocolos Laboratoriais. 2ª edição, 2005, Edições Universidade Fernando Pessoa.
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Teaching Methodologies and Assessment Criteria |
To know:
- Classes will consist of 2 components: theoretical (CT, 2h - theoretical course) and laboratory (CL, 2h). Theoretical classes bring together all the components inherent to “knowing” as content. Theoretical sessions should be followed up by the students later using the slides of the subject and, above all, the reference literature pointed out by the teacher.
Know how to do:
- CL classes will be used for the following purposes: (i) extension of T classes, to consolidate the knowledge taught using visualization and practical experimentation; (ii) learning and training of simple and frequent laboratory techniques used in Microbiology.
Knowing how to be:
- Attendance at CL presupposes the mandatory use of a gown that the students must accompany and disposable gloves that will be made available in the laboratory.
Theoretical assessment of knowledge, in periodic forma
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Language |
Portuguese. Tutorial support is available in English.
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