| Code |
13442
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| Year |
1
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| Semester |
S2
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| ECTS Credits |
6
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| Workload |
PL(30H)/T(30H)
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| Scientific area |
Biochemistry
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Entry requirements |
non applicable
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Mode of delivery |
Face-to-Face
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Work placements |
Not aplicable
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Learning outcomes |
1. To acquire general knowledge in microbiology and to know the microorganisms taxonomic position;
2. To know structural differences between microorganisms and their nutritional, growth and reproduction requirements;
3. To know the most important microorganisms related to human diseases and their mechanisms of pathogenicity: bacteria, fungi, virus and parasites;
4. To associate microorganisms to the caused infection and to know its implication in the human organism;
5. To know for each microorganism their characteristics implicated in investigation studies;
6. To recognize the importance of some microorganisms in the biotechnology development;
7. To understand and to perform laboratorial procedures commonly used in diagnosis and investigation microbiology.
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Syllabus |
THEORY:
1. Introduction; Taxonomy; Pathogenicity; vaccines; laboratorial diagnosis;
2. Bacteriology: anatomy, physiology, growth and replication; genetic; biotechnology; microorganisms and infections, preventing and treatment;
3. Parasitology: life cycle; pathogenicity and laboratorial diagnosis; Protozoa and Methazoa;
4.Micology: nutrition, metabolism and reproduction; Mucocutaneous and systemic mycoses;
5. Virology – replication, infections; diagnosis methods; DNA and RNA virus.
LABORATORIAL: Introduction to microbiology laboratory; safety rules; sterilization processes. Optical microscopy. Observation of bacteria and yeast slides. Culture media: types and preparation. Aseptic technique. Microorganisms manipulation and isolation. Slides preparation. Simple and Gram stain method; Ziehl Neelsen (observation); Capsule and spores stain. Bacterial morphology. Microorganisms mobility. Growth curve Biochemical tests. Antibiotic susceptibility determination. PCR.
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Main Bibliography |
- João Carlos F. de Sousa, Wanda Canas Ferreira, Nelson Lima. Microbiologia 2010, Lidel
- Wanda Ferreira e João Carlos F de Sousa. Microbiologia Volumes II e III 2007 Lidel
- João Carlos Sousa, Cristina Abreu, Fátima Cerqueira. Microbiologia: Protocolos Laboratoriais. 2ª edição, 2005, Edições Universidade Fernando Pessoa.
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Language |
Portuguese. Tutorial support is available in English.
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