Biochemistry I

Code	13540
Year	2
Semester	S1
ECTS Credits	6
Workload	PL(15H)/T(30H)/TP(15H)
Scientific area	Biochemistry
Entry requirements	Not applicable
Mode of delivery	Face to Face with recourse to e-learning.
Work placements	Not applicable.
Learning outcomes	This Course aims to provide students with a set of knowledge in the area of basic biochemistry that enable them to understand the molecular logic of life and apply them to Biomedical Sciences. At the end of this course the student should be able to: -Identify the general principles and terminology of Biochemistry; -Give examples of pathologies associated with changes in the structure and/or concentration of biomolecules; -Recognize and describe the structure and properties of biomolecules; -Explain the relation between the structure and properties of biomolecules and their biological function; -Identify and describe the basic principles of enzymology and enzyme mechanisms; -Apply the acquired knowledge to interpret and to solve problems in the fields of Biochemistry and Biomedical Sciences; -Perform experimental techniques in the fields of Biochemistry and Biomedical Sciences and analyze and interpret the results accurately and critically.
Syllabus	1-INTRODUCTION TO BIOCHEMISTRY. Origin of biomolecules. The water molecule (properties and buffer solutions). 2-PROTEINS. Structure and properties of amino acids. Stereochemistry and acid-base properties. Hierarchy and structural classification of proteins. Structure and function of proteins. Allosteric proteins: hemoglobin. Purification and characterization of proteins. Properties of enzymes. 3-CARBOHYDRATES. Structure and general properties. Stereoisomerism, mutarotation and anomeric forms. 4-LIPIDS. Classification, structure and properties. 5-NUCLEIC ACIDS. Structure, properties and function. Plasmids. 6-VITAMINS AND COENZYMES. General features of water-soluble and fat soluble vitamins. Structure and biological role of vitamins. Coenzyme forms. 7-EXPERIMENTAL Preparation and testing of buffer solutions. Potentiometric titration of amino acids. Bradford Method and kinetics of the enzyme invertase Hemoglobin Cholesterol quantification
Main Bibliography	1- D.L. Nelson e M.M. Cox (2017) Lehninger Principles of Biochemistry: International Edition, 7th ed., W.H.Freeman & Co Ltd, NY, USA, ISBN 1464126119 2- J.M. Berg, J.L. Tymoczko , L. Stryer (2007) Biochemistry. 6th ed., Freeman, NY, USA, ISBN 0716787245 3- A. Quintas, A.P Freire, M.J. Halpern , (2008) Bioquímica Organização Molecular da Vida, Ed. Lidel, Lisboa, Portugal, ISBN 9789727574315 4- 4- D. Voet, J.G. Voet (2004). Biochemistry. 3ª ed.,New York: J. Wiley & Sons.
Language	Portuguese. Tutorial support is available in English.