| Code |
13543
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| Year |
2
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| Semester |
S2
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| ECTS Credits |
6
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| Workload |
PL(15H)/T(30H)/TP(15H)
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| Scientific area |
Biochemistry
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Entry requirements |
Not applicable.
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Mode of delivery |
Face to face.
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Work placements |
Not applicable.
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Learning outcomes |
The unit of Biochemistry II aims to develop and apply the knowledge, attitudes and skills acquired in Biochemistry I, in understanding the biochemical meaning of metabolic strategies and its regulation.
At the end of this course the student should be able to:
-Describe and to explain the bioenergetic aspects related to oxidative phosphorylation, the substrate-level phosphorylation and the metabolism of the main nutritional components of living beings;
-Identify, to describe and to interpret the metabolic pathways of carbohydrates, lipids, amino acids and nucleotides;
-Compare the role of different hormones in the energy metabolism and to interpret the mechanism of hormonal regulation;
-Explain the integration of metabolic pathways and its regulation;
-Perform experimental techniques in the field of Biochemistry and to analyze and interpret the results accurately and critically;
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Syllabus |
1. Metabolism and Bioenergetics. Oxidation-reduction reactions in biological systems.
2. Carbohydrate metabolism. Glycolysis. Regulation. Glycogen metabolism. Gluconeogenesis. Precursors and Regulation. Pentose phosphate pathway.
3. Citric acid cycle. Regulation.
4. Oxidative phosphorylation. Electron transport chain. Regulatory phosphorylation
5 . Lipid metabolism. ß-oxidation of fatty acids. Metabolism of ketone bodies. Synthesis of lipids. Cholesterol and lipoproteins metabolism
6. Metabolism of amino acids. Urea cycle.
7. Metabolism of nucleic acids
8. Hormonal regulation and integration of mammalian metabolism
9. Experimental: Hydrolysis of compounds containing phosphate groups. Production of pyruvate and acetaldehyde during fermentation of glucose by yeast. Mitochondrial respiration. Amino acid metabolism: determination of alanine transaminase activity.
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Main Bibliography |
- D.L. Nelson e M.M. Cox (2021) Lehninger Principles of Biochemistry: International Edition, 8th ed., W.H.Freeman & Co Ltd.
- J.M. Berg, J.L. Tymoczko, L. Stryer (2015) Biochemistry. 8th ed., New York: Freeman
Complementar
- A.Quintas, A.P Freire, M.J. Halpern, (2008) Bioquímica - Organização Molecular da Vida, Ed.Lidel
- D. Voet, J.G. Voet (2010). Biochemistry. 4th ed., New York: John Wiley & Sons
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Teaching Methodologies and Assessment Criteria |
Biochemistry II Teaching and Learning Assessment (CEA): 70% Theoretical grade (3 written tests + mini-tests) + 30% Practical grade.
Minimum CEA required to pass the Course Unit: 10 points.
Minimum CEA required for admission to the final exam – approval in the practical component (minimum grade of 9.5 points).
A.1 The Theoretical Assessment will be carried out through 3 written tests (90%) and theoretical mini-tests (10%). There is no minimum grade required for each individual test; however, an average minimum grade of 9.5 points in the theoretical assessment is required to pass this component. 1st Partial Theoretical Test (30%) – April 13, 18:30; 2nd Partial Theoretical Test (30%) – May 18, 18:30; 3rd Global Theoretical Test (30%) – June 8, 18:30. Additional theoretical mini-tests will be carried out at the end of theoretical classes (online or on paper), without prior notice (between 4 and 6), covering the main topics taught.
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Language |
Portuguese. Tutorial support is available in English.
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