| Code |
13543
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| Year |
2
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| Semester |
S2
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| ECTS Credits |
6
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| Workload |
PL(15H)/T(30H)/TP(15H)
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| Scientific area |
Biochemistry
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Entry requirements |
Not applicable.
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Mode of delivery |
Face to face.
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Work placements |
Not applicable.
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Learning outcomes |
The unit of Biochemistry II aims to develop and apply the knowledge, attitudes and skills acquired in Biochemistry I, in understanding the biochemical meaning of metabolic strategies and its regulation.
At the end of this course the student should be able to:
-Describe and to explain the bioenergetic aspects related to oxidative phosphorylation, the substrate-level phosphorylation and the metabolism of the main nutritional components of living beings;
-Identify, to describe and to interpret the metabolic pathways of carbohydrates, lipids, amino acids and nucleotides;
-Compare the role of different hormones in the energy metabolism and to interpret the mechanism of hormonal regulation;
-Explain the integration of metabolic pathways and its regulation;
-Perform experimental techniques in the field of Biochemistry and to analyze and interpret the results accurately and critically;
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Syllabus |
1. Metabolism and Bioenergetics. Oxidation-reduction reactions in biological systems.
2. Carbohydrate metabolism. Glycolysis. Regulation. Glycogen metabolism. Gluconeogenesis. Precursors and Regulation. Pentose phosphate pathway.
3. Citric acid cycle. Regulation.
4. Oxidative phosphorylation. Electron transport chain. Regulatory phosphorylation
5 . Lipid metabolism. ß-oxidation of fatty acids. Metabolism of ketone bodies. Synthesis of lipids. Cholesterol and lipoproteins metabolism
6. Metabolism of amino acids. Urea cycle.
7. Metabolism of nucleic acids
8. Hormonal regulation and integration of mammalian metabolism
9. Experimental: Hydrolysis of compounds containing phosphate groups. Production of pyruvate and acetaldehyde during fermentation of glucose by yeast. Mitochondrial respiration. Amino acid metabolism: determination of alanine transaminase activity.
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Main Bibliography |
- D.L. Nelson e M.M. Cox (2021) Lehninger Principles of Biochemistry: International Edition, 8th ed., W.H.Freeman & Co Ltd.
- J.M. Berg, J.L. Tymoczko, L. Stryer (2015) Biochemistry. 8th ed., New York: Freeman
Complementar
- A.Quintas, A.P Freire, M.J. Halpern, (2008) Bioquímica - Organização Molecular da Vida, Ed.Lidel
- D. Voet, J.G. Voet (2010). Biochemistry. 4th ed., New York: John Wiley & Sons
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Teaching Methodologies and Assessment Criteria |
Teaching–Learning Assessment (TLA): 70% Theoretical (T) + 30% Practical (P)
Theoretical assessment:
T1 (30%) + T2 (30%) + T3 (30%) + mini-tests at the end of theoretical classes (10%)
In the multiple-choice questions of the 3 tests and the exams, a 10% penalty of the question’s value will be applied for each incorrect answer.
Practical assessment:
TP1 (50%) + TP2 (50%) based on the P laboratory work. If the average of the two TP tests is below 9.5/20, a recovery test may be taken, with a maximum grade of 9.5/20 in the Practical component. Minimum grade for approval: 9.5/20 in T and 9.5/20 in P.
Course approval or admission to the final exam: minimum of 9.5/20 in P. The final exam assesses the Theoretical component and corresponds to 70% of the final grade, while the Practical component grade (30%) is maintained.
Attendance at T and P/TP classes is mandatory. A maximum of 3 absences in T classes and 1 absence in P/TP classes is allowed; failure to comply prevents admission to the exam.
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Language |
Portuguese. Tutorial support is available in English.
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