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Analytical Methods II

Code 13651
Year 2
Semester S2
ECTS Credits 6
Workload PL(30H)/T(30H)
Scientific area Physics and Chemistry
Entry requirements No previous requirements.
Mode of delivery Face-to-face
Work placements Not applicable.
Learning outcomes To explore the analytical methods used in analytical chemistry and biochemistry, with emphasis on chromatography and electrophoresis, electrochemical methods, methods of atomic spectroscopy and radiochemical methods as well as their equipment. The course will also explore experimental design.
At the end of this curriculum unit the student should be able to:
- Explain the principles and applications of different types of electrophoresis
- Explain the basic principles and mode of operation of equipment used in absorption spectroscopy / atomic emission and select the most appropriate for solving a real case.
- Explain the basic principles of operation of equipment commonly used in electrochemical methods and select the most suitable for a study case.
- Explain the principles of chromatography, GC-MS and HPLC.
- Explain the basic principles of radiochemical methods.
- Apply experimental design to experiments.
Syllabus Lectures
1-Chromatografic methods
1.1-GC-MS
1.2-HPLC
2- Electrophoresis
3- Atomic spectroscopic methods
4- Electroanalytical methods
4.1- Introduction to electroanalytical methods
4.2- Potentiometric methods
4.3- Voltammetric methods
5- Radiochemical methods
6- Experimental design
Laboratory classes
- Determination of metals by atomic absorption
- Conductimetry: Study of the behavior of strong electrolytes and conductimetric titration
- Potentiometric Determination of iron with potassium dichromate
- Determination of enzyme activity of the soluble form of human catechol-O-methyltransferase (hSCOMT) using HPLC reverse phase chromatography.
- Nucleic acid electrophoresis on agarose gel
Main Bibliography - J.N. Miller, J.C. Miller,” Statistics and Chemometrics for Analytical Chemistry”, 6th Ed.,Prentice Hall, 2010.
- D.A. Skoog, F.J. Holler, S.R. Crounch, “Principles of instrumental analysis, 6th edition”, Thomson Learning, 2007.
- D.C. Harris, “Quantitative Chemical Analysis”, 7ª Ed., W. H. Freeman and Company, 2007.
- D.A. Skoog, D. West, F.J. Holler, S.R. Crounch, “Analytical Chemistry. An Introduction”, 7th Ed., Thomson Learning, 2000
- D. Harvey, “Modern Analytical Chemistry”, 2nd Ed., McGraw-Hill, 2000.
- F.W. Fifield, D. Kealey, “Principles and Pratice of Analytical Chemistry”, 4th Ed., Chapman & Hall,1995.

Teaching Methodologies and Assessment Criteria Teaching in this curricular unit is structured into theoretical and laboratory classes. Lectures are taught with the help of audio-visual media and give an overview of the techniques studied, with emphasis on understanding the most important underlying concepts. Throughout the semester students have laboratory classes using instrumental methods. There are also practical lessons where knowledge is developed through problem solving.
The evaluation will tend to be continuous, and take into account two factors of evaluation: 30% Pratical + 70% Theoretical.
where:
T-score of 3 partial tests or final exam;
P- laboratory performance evaluation, pre-lab questions and reports.
They must carry out all laboratory work.
The grade of the theoretical-practical component must be equal to or greater than 9.5 values to obtain a course frequency, otherwise the student will not be admitted.
Language Portuguese. Tutorial support is available in English.
Last updated on: 2021-06-10

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