| Code |
15037
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| Year |
1
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| Semester |
S2
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| ECTS Credits |
5
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| Workload |
TP(40H)
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| Scientific area |
Biomedicina/Medicina/Bioquímica
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Entry requirements |
Without entry requirements
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Mode of delivery |
Face to face.
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Learning outcomes |
In cell NMR: principles, methods and applications.
At the end of the course unit the learner is expected to be able to: (i) understand the fundamental concepts of NMR and its application in different areas of research
(i) understand the fundamental concepts of NMR and its application in different areas of research
(ii) to perform NMR studies on cells, immobilise cells in agarose in the NMR tube, set up the perfusion system of cells with medium in the NMR tube and acquire samples with cells;
(iii) determine metabolites and cell viability throughout the NMR experiment;
(iv) to use the NMR spectrometers from CICS-UBI belonging to the national PTNMR network.
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Syllabus |
The contents of the advanced in cell NMR course involves sample preparation with cells and cell perfusion system using a bioreactor in NMR tube, NMR analysis using 1D experiments according to the complexity of the sample in order to perform a qualitative and quantitative study of metabolites and cell viability.
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Main Bibliography |
Barbieri, L., Luchinat, E., & Banci, L. (2016). Characterization of proteins by in-cell NMR spectroscopy in cultured mammalian cells. Nature protocols, 11(6), 1101–1111. https://doi.org/10.1038/nprot.2016.061
Luchinat, E., & Banci, L. (2018). In-Cell NMR in Human Cells: Direct Protein Expression Allows Structural Studies of Protein Folding and Maturation. Accounts of chemical research, 51(6), 1550–1557. https://doi.org/10.1021/acs.accounts.8b00147
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Teaching Methodologies and Assessment Criteria |
The methodologies are: hands-on sessions using cell extracts in the NMR tube; perfusion system with bioreactor coupled to the NMR tube; acquisition 31P spectra, processing and analysis using topspin.
The teaching of this advanced course will be centred in the student, and will have a theoretical and practical component whose active participation in the learning process will allow a further development of his capacities of reasoning, search for scientific information, self-learning and self-evaluation. Learning is developed in small groups, with the use of appropriate resources for self-learning and involves the integration of information from current scientific research.
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Language |
Portuguese. Tutorial support is available in English.
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