Code |
15044
|
Year |
1
|
Semester |
S2
|
ECTS Credits |
5
|
Workload |
TP(40H)
|
Scientific area |
Biomedicina/Medicina/Bioquímica
|
Entry requirements |
Without entry requirements
|
Learning outcomes |
Upon completion of the course, the student should: - Have an overview of several basic and advanced techniques of fluorescence microscopy used in biomedical research. - Have acquired fundamental notions on image analysis, such as digitizing, image enhancement, segmentation, and feature classification.
- Know how to select the image acquisition mode suitable for the intended purpose. - Perform manual and automatic measurements using image analysis tools. - Perform object tracking.
-Understand the applications and limitations of each technique. - Know and apply the fundamentals of ethics in Image analysis.
|
Syllabus |
- Basic concepts in fluorescence microscopy: different fluorochromes, excitation methods, emission characteristics, purpose, and uses. - Basic concepts about digital images. - Basic methods of computerized image analysis - Multidimensional data (2D, 3D, time-lapse, position) - Image processing in preparation for analysis, image enhancement - Object/particle analysis, intensity, and morphometric measurements - Tracking motion of cells or particles - Extraction of quantitative data - Automatic analysis of large data sets
- The course will also address ethical issues associated with the proper acquisition and analysis of images. - The course also introduces various freely available software tools (ImageJ/Fiji) and some commercial software (Imaris).
|
Main Bibliography |
Fluorescence Microscopy: From Principles to Biological Applications, 2nd Edition (2017) Ulrich Kubitscheck (Editor) ISBN: 978-3-527-33837-5 Wiley-Blackwell
Bioimage Data Analysis, Edited by Kota Miura, Wiley-VCH ePDF ISBN: 978-3-527-80092-6
Quantitative Imaging in Cell Biology, Methods in Cell Biology, Volume 123 (2014) Editors Jennifer C. Waters & Torsten Wittmann
|
Language |
Portuguese. Tutorial support is available in English.
|