| Code |
15906
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| Year |
1
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| Semester |
S1
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| ECTS Credits |
3
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| Workload |
PL(15H)/T(15H)
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| Scientific area |
Chemistry
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Entry requirements |
- Holders of an academic degree recognized by the course coordination as meeting the necessary competencies for admission to the course;
- Holders of an academic, scientific, or professional curriculum recognized by the course coordination as demonstrating the ability to complete the course.
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Learning outcomes |
The course ‘Development of the Preparative Chromatography Process’ is an initiative carried out under the UBImpulso Adultos project, funded by the Recovery and Resilience Plan (RRP/PRR), within the Next Generation EU mechanism of the European Union, and is taught by the Faculty of Sciences.
The Advanced Course is aimed at professionals in the biotechnology industry involved in biomolecule purification, as well as master’s and PhD students, postdoctoral researchers, and research laboratory technicians interested in the field.
The course aims to integrate the theory and practice of preparative chromatography of biomolecules, promoting an in-depth understanding of process principles and their application in the development of solutions for the pharmaceutical industry.
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Syllabus |
1 – Processing of Biotechnology-Derived Products:
• The role of chromatography in the downstream stage
• Bioproducts, their contaminants, and purity criteria
• Physicochemical properties of biomolecules (UV absorbance, size, charge, hydrophobicity, solubility, mechanical and chemical stability, viscosity, diffusivity).
2 – Columns and Chromatographic Equipment at Laboratory Scale:
• The chromatographic system
• Definitions of velocity and pressure in a chromatographic column
• Correction of extra-column effects
• Packing a chromatographic column (practical session)
3 – Adsorption Equilibrium:
• Adsorption isotherms. Method for obtaining them
• Stoichiometric displacement model
• Steric mass action model
4 – Microcalorimetry as a Tool in Preparative Chromatography:
• Versatility and potential
• Thermodynamic perspective (practical session in flow microcalorimetry)
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Main Bibliography |
- Carta, G. and Jungbauer, A. (2020) Protein Chromatography. Process Development and Scale-Up, 2nd Edition, Wiley-VCH: Weinheim, Germany.
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Teaching Methodologies and Assessment Criteria |
Open-book test, graded on a 0–20 scale.
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Language |
Portuguese. Tutorial support is available in English.
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