| Code |
15982
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| Year |
2
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| Semester |
S2
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| ECTS Credits |
6
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| Workload |
PL(15H)/T(30H)/TP(15H)
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| Scientific area |
Chemistry
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Entry requirements |
-
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Learning outcomes |
The Instrumental Analysis Methods course aims to provide general basic knowledge of the instrumental techniques of analysis usually used in chemical analysis laboratories, namely, the UV-Vis molecular spectrophotometry, atomic absorption/emission spectroscopy, electroanalytical and chromatographic methods.
At the end of this course the students should be able to:
-know the basic principles of the equipment operation and know to use properly some of the most used in chemical analysis laboratories.
- assess the benefits and limitations of different instrumental methods
- select an appropriate instrumental method to solve a particular problem in a case study, based on theoretical knowledge and using scientific literature as a resource.
- interpret, explain and express accurately the analytical results.
- be familiar with the analytical methods validation procedures.
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Syllabus |
1. Introduction to Instrumental Methods of Analysis.
Analytical methods classification. Introduction to statistical treatment of analytical data. Standardizing methods: linear regression and calibration curves. Brief introduction to analytical methods validation.
2. UV-Vis Absorption Spectrophotometry
Introduction. Chromophore. Beer Law. Instrumentation. Qualitative and Quantitative analysis.
3. Atomic Absorption/Emission Spectroscopy
Flame atomic absorption spectroscopy. Electrothermal atomic absorption spectroscopy. Flame atomic emission spectroscopy. Brief introduction to ICP and ICP-MS.
4. Electroanalytical Methods
Review of electrochemical basic concepts. Potentiometric Methods: Ion-selective electrodes. Voltammetric methods.
5. Chromatographic Methods
Introduction and chromatographic classification. Theoretical fundaments. Optimization of a chromatographic separation. Liquid chromatography, HPLC. Gas chromatography.
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Main Bibliography |
D.A. Skoog, D. West, F.J. Holler, S.R. Crounch (2000). “Analytical Chemistry. An Introduction”, 7th ed., Thomson Learning.
F. Rousseac, A. Rousseac (2007), Chemical Analysis: Modern Instrumentation Methods and Techniques, 2nd ed., Wiley.
D. Harris (2008). Analise Química Quantitativa, 7ª ed., LTC.
G.D. Christian (2004). Analytical Chemistry.6th ed., Wiley.
M. L. S. Gonçalves (2001). Métodos Instrumentais para Análise de Soluções: Análise Quantitativa, 4ª ed., Fundação Calouste Gulbenkian.
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Teaching Methodologies and Assessment Criteria |
The final grade (FG) for the course unit is calculated according to the following formula:
FG = 0.60 × T + 0.10 × S + 0.30 × P
where:
T – Average of the grades obtained in the three assessment tests or the grade obtained in the final examination.
S – Group oral presentation of a seminar.
P – Grade of the practical component (laboratory and theoretical-practical classes).
Component P results from the weighting of continuous assessment (7.5%), individual resolution of questions at the beginning of laboratory sessions (7.5%), processing of results and preparation of the report (25%), and the practical knowledge assessment test (60%).
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Language |
Portuguese. Tutorial support is available in English.
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