| Code |
16813
|
| Year |
1
|
| Semester |
L0
|
| ECTS Credits |
1
|
| Workload |
O (18H)/PL(10H)
|
| Scientific area |
Biomedicina
|
|
Entry requirements |
Bachelor's degree or legal equivalent
|
|
Learning outcomes |
On completion of the course, the student should:
- Know the various basic and advanced fluorescence microscopy techniques used in biomedical research.
- Know how to select the appropriate image acquisition mode for the intended purpose.
- Understand the applications and limitations of each technique.
|
|
Syllabus |
Introduction to Fluorescence Microscopy
Optical Sectioning
Live Cell Imaging
Hands-On Microscopes (Axio Observer Z1, Axio Imager Z2, Confocal LSM 710)
|
|
Main Bibliography |
- Fluorescence Microscopy: From Principles to Biological Applications, 2nd Edition. Ulrich Kubitscheck (Editor) ISBN: 978-3-527-33837-5 June 2017 Wiley-Blackwell
- Bioimage Data Analysis, Edited by Kota Miura, Wiley-VCH. ePDF ISBN: 978-3-527-80092-6
- Quantitative Imaging in Cell Biology, Methods in Cell Biology, Volume 123 (2014). Editors Jennifer C. Waters & Torsten Wittmann
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|
Teaching Methodologies and Assessment Criteria |
The course consists of hands-on practical sessions on the microscopes assigned to the microscopy laboratory. Assessment involves the individual application of knowledge in contexts that students will be able to transpose to their research projects.
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|
Language |
Portuguese. Tutorial support is available in English.
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