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Advanced Courses II

Advanced Courses II

Code	16860
Year	1
Semester	S2
ECTS Credits	5
Workload	TP(40H)
Scientific area	Biomedicina/Medicina/Bioquímica
Entry requirements	-
Learning outcomes	The objective of this course is to provide students with advanced knowledge on the application of Nuclear Magnetic Resonance (NMR) spectroscopy in the structural and functional characterization of eukaryotic and prokaryotic secretomes. Students are expected to develop skills in the interpretation of spectroscopic data, identification of secreted biomolecules, and understanding the role of these molecules in biological and pathological processes. In addition, students will acquire the ability to design and implement experimental strategies based on NMR for the analysis of metabolites present in microbial and cellular secretomes. The learning objectives will be achieved through a combination of theoretical and practical sessions focused on spectroscopic analysis, and case studies. The pedagogical approach emphasizes the integration of theoretical concepts with experimental applications, promoting students’ critical ability to interpret NMR data in complex biological systems.
Syllabus	The course contents are structured to ensure a logical progression from the theoretical foundations of NMR spectroscopy to its practical applications in the study of biological secretomes. The introduction to the principles of NMR provides the conceptual basis necessary for understanding the spectroscopic techniques used in the characterization of biomolecules. Subsequently, the discussion of experimental methods and sample preparation enables students to understand the key steps required for the analysis of biological systems. The inclusion of multidimensional techniques and spectroscopic data analysis tools contributes to the development of skills in the interpretation of NMR spectra. Finally, the analysis of real examples and applications of NMR in eukaryotic and prokaryotic secretomes allows students to consolidate the acquired knowledge and develop an integrated understanding of the potential of this technique in biomedical and biotechnological research.
Main Bibliography	Claridge, T. D. W. (2016). High-Resolution NMR Techniques in Organic Chemistry. Elsevier. Cavanagh, J., Fairbrother, W., Palmer, A., Rance, M., & Skelton, N. (2007). Protein NMR Spectroscopy: Principles and Practice. Academic Press. Keeler, J. (2010). Understanding NMR Spectroscopy. Wiley. Sattler, M., Schleucher, J., & Griesinger, C. (1999). Heteronuclear multidimensional NMR experiments for the structure determination of proteins in solution. Progress in Nuclear Magnetic Resonance Spectroscopy, 34, 93–158. Emwas, A. H. et al. (2019). NMR spectroscopy for metabolomics research. Metabolites, 9, 123. Markley, J. L. et al. (2017). The future of NMR-based metabolomics. Current Opinion in Biotechnology, 43, 34–40.
Language	Portuguese. Tutorial support is available in English.