| Code |
17228
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| Year |
1
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| Semester |
S1
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| ECTS Credits |
6
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| Workload |
TP(60H)
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| Scientific area |
Biochemistry
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Entry requirements |
Not aplicable
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Learning outcomes |
THEORETICAL 1. Molecules of Molecular Biology: nucleic acids, proteins. Prokaryotic and eukaryotic genomes. 2. Isolation of DNA and RNA. 3. Nucleic acid manipulation: Electrophoresis; PCR; Nucleases and modification enzymes; Cloning vectors and recombinant molecules; DNA sequencing; Identification of gene banks. 4. Gene and protein expression: RT-PCR and real-time PCR; Hybridization, blotting, in situ hybridization; Subtractive hybridization and DNA microarrays; Next-generation RNA sequencing, Western blot; immunocytochemistry. 5. Applications of Molecular Biology: Genetic markers; DNA fingerprinting and forensic science; Nucleic acid analysis and molecular diagnostics; Small RNAs; Genome editing - CRISPR-Cas technology. PRACTICE (laboratory classes): isolation of total RNA from rat liver; cDNA synthesis; PCR/electrophoresis; Cloning into a vector suitable
for PCR products; E. coli transformation; pDNA isolation; Restriction/digestion product analysis. Sequencing.
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Syllabus |
THEORETICAL 1. Molecules of Molecular Biology: nucleic acids, proteins. Prokaryotic and eukaryotic genome 2. Isolation of DNA and RNA 3. Manipulation of nucleic acids: Electrophoresis; PCR; Nucleases and modification enzymes; Cloning vectors and recombinant molecules; DNA sequencing; Identification of gene banks 4. Expression of genes and proteins: RT-PCR and real-time PCR; Hybridization, blotting, in situ hybridization; Subtractive hybridizations and DNA microarrays; Next-generation RNA sequencing, Western blot; immunocytochemistry 5. Applications of Molecular Biology: Genetic markers; DNA fingerprinting and forensic science; Nucleic acid analysis and molecular diagnostics; Small RNAs; Genome editing - CRISPR-Cas technology. PRACTICE (laboratory classes): isolation of total RNA from rat liver; cDNA synthesis; PCR/electrophoresis; Cloning of vector suitable for PCR products; E. coli transformation; pDNA isolation; Restriction/digestion product analysis. Sequencing
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Main Bibliography |
1. Main Bibliography
- Benjamin A. Pierce. "Genetics - A Conceptual Approach," 7th Edition, 2020. W. H. Freeman Publishers
- Lodish H, Berk A, Zipursky SL, et al. "Molecular Cell Biology." 4th or 5th Ed. New York: W. H. Freeman, 2021
- Alberts B., Bray D., Hopkin K. et al., "Fundamentals of Cell Biology." 4th Ed. Artmed, 2017.
2. Complementary Bibliography
- José Luque, Angel Herraez, "Molecular Biology and Genetic Engineering" - Concepts, Techniques, and Applications in Health Sciences. Elsevier, 2006
- Carlos Azevedo, “Cellular and Molecular Biology,” 4th Ed., Lidel, 2005
- “Cell Biology,” 3rd Ed.; A Laboratory Manual; Edited by: Julio E. Celis, Copyright, Elsevier Inc., 2006
- PubMed: http://www.ncbi.nlm.nih.gov/pubmed/
- Blast: http://blast.ncbi.nlm.nih.gov/Blast.cgi
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Teaching Methodologies and Assessment Criteria |
Theoretical classes consist of an oral presentation of the subject using a projector, with intense student-teacher interaction. To ensure student participation and motivation, discussion between students and the teacher is encouraged. In practical classes, students perform some basic molecular biology techniques, allowing them to understand all of its applications. They also solve exercises related to all content and will have two bioinformatics classes (theoretical and practical).
ASSESSMENT CRITERIA:
Theoretical Component (CT, 80%): Corresponds to the grade on a comprehensive written test.
Practical Component (CP, 20%):Corresponds to the grade on the report on the project developed in the practical classes. Attendance score = 0.8 x CT + 0.2 x CP
FINAL EXAM:Assessment of theoretical, practical, and theoretical-practical content. Students are only admitted to the exam if they attend the practical and theoretical-practical classes. Students who take the improvement exam will receive
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Language |
Portuguese. Tutorial support is available in English.
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