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Chemical and Biological Analysis for Clinical Application

Code 8440
Year 1
Semester S2
ECTS Credits 6
Workload PL(30H)/T(30H)
Scientific area Biochemistry
Entry requirements Not applicable.
Mode of delivery Face-to-face
Work placements --
Learning outcomes To prepare student for the first contact with Clinical Laboratory (CL)
To give students theoretical/practical training in the areas of Clinical Analysis
To provide clinical and laboratory knowledge for integration in specific teams
To perform basic training in expertise areas
At the end of this course the student should be able to:
-Know the hierarchy of a CL
-Apply knowledge in the understanding of the disease
-Perform laboratory techniques with increasing complexity
-Acquire practical skills including microscopy
-Interpret the results of the External Quality Assessment
-Know how to work and apply the SPSS software and statistical methods to evaluate the quality
-Identify and recognize changes in analytical parameters
-Interpret an analytical request and know how to fit the laboratory findings in different pathologies
-Identify laboratory cases of urgency
-Know to use scientific reasoning in the search for self-learning
Syllabus UP1-1 LAC: Evolution/Quality
1.2Erythrocyte, leukocyte, platelet: associated diseases
1.3Hemostasis: evaluation
2Clinical Chemistry: Microbiology, Immunology, Endocrinology:
2.1Diabetes: changes in Renal/Lipidic functions in a patient
2.2Diabetes and CVD markers
2.4Renal funtion
2.5Urinary tract infection
2.6Renin-angiotensin-aldosterone system
2.7Other regulatory hormones
2.8Hepatitis: Etiology/diagnosis. Importance of hepatitis, cytomegalovirus, rubella, toxoplasmosis in pregnant women
3Tumor markers
UP3-SPSS: Theoretical-pratical application
Interpretation of analytical request
Blood count and platelets: automatic/manual counting
Description of Wintrobe and Westergreen techniques
Execution of blood smears
Differential leukocyte and reticulocyte count
Quantification of HbA1c and thyroid hormones
Urinalysis and bacteriological exam
Interpretation of different outcomes and resolution of practical cases
Main Bibliography Basic Bibliography:
-McPherson RA; Pincus MR. Henry’s Clinical Diagnosis and Management by Laboratory Methods, 22th Edition. Saunders, Elsevier (2012).
-HillmanRS; Ault KA; Rinder H. Hematology in Clinical Practice, 4th Edition. McGraw-Hill, USA, (2005)
-Guyton AC; Hall JE. Tratado de Fisiologia Médica, 11ªEdição. Editora: Elsevier (2006)

Complementar reading:
Bishop, LM; Fody, PE; Schoeff L. Clinical Chemistry: Principles, Procedures, Correlations, 5th Edition. Lippincott Williams and Wilkins, Baltimore (2005)
Teaching Methodologies and Assessment Criteria The student acquires the competences proposed through theoretical classes and theoretical-practical classes of dynamic character (classes by videoconference) in order to interact both in the interpretation of analytical bulletins, and resolution of practical cases, understanding of methodologies used in the quantification of some parameters, as well as the entire process of interpreting laboratory results and their validation. Whenever appropriate, appropriate photographs will be shown, especially with regard to microscopic observations. Classification 70% Theoretical (T), and 30% Theoretical-Practical (TP). The TP evaluation includes the realization of works carried out during the TP classes, proposed for evaluation (10%) and the elaboration and discussion of a theme monograph. previously selected and / or presentation and discussion of a scientific article. (20%). The minimum grade for approval is 9.5 values.
Language Portuguese. Tutorial support is available in English.
Last updated on: 2020-05-14

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