| Code |
12813
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| Year |
2
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| Semester |
S1
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| ECTS Credits |
6
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| Workload |
PL(15H)/T(30H)/TP(15H)
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| Scientific area |
Biochemistry
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Entry requirements |
Not applicable.
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Mode of delivery |
Face to face lectures.
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Work placements |
Not applicable
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Learning outcomes |
This Course aims to provide students with a set of knowledge in the area of basic biochemistry that enable them to understand the molecular logic of life.
At the end of this course the student should be able to:
-Identify the general principles and terminology of Biochemistry;
-Recognize and describe the structure and properties of biomolecules;
-Explain the relation between the structure and properties of biomolecules and their biological function;
-Identify and describe the basic principles of enzymology and enzyme mechanisms;
-Apply the acquired knowledge to interpret and to solve problems in the field of Biochemistry;
-Perform experimental techniques in the field of Biochemistry and analyze and interpret the results accurately and critically
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Syllabus |
1 INTRODUCTION TO BIOCHEMISTRY. Origin of biomolecules. The water molecule (properties and buffer solutions).
2 PROTEINS. Structure and properties of amino acids. Stereochemistry and acid-base properties. Hierarchy and structural classification of proteins. Structure and function of proteins. Allosteric proteins: hemoglobin. Purification and characterization of proteins. Properties of enzymes.
3-CARBOHYDRATES. Structure and general properties. Stereoisomerism, mutarotation and anomeric forms.
4. LIPIDS. Classification, structure and properties.
5. NUCLEIC ACIDS. Structure, properties and function. Plasmids.
6. VITAMINS AND COENZYMES. General features of water-soluble and fat soluble vitamins. Coenzyme forms.
EXPERIMENTAL
Preparation and testing of biological buffer solutions
Determination of protein concentration by Bradford method
Study of the kinetics of sucrose hydrolysis by the enzyme invertase
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Main Bibliography |
Required Reading
- D.L. Nelson e M.M. Cox (2017) Lehninger, Principles of Biochemistry, 7 ed., Worth Publishers.
- A.Quintas, A.P Freire, M.J. Halpern, (2008) Bioquímica - Organização Molecular da Vida, Ed. Lidel
- D. Voet, J.G. Voet (2010). Biochemistry. 4ª ed.,New York: J. Wiley & Sons.
- J.M. Berg, J.L. Tymoczko, L. Stryer (2011) Biochemistry. 7ª ed., New York: Freeman
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Teaching Methodologies and Assessment Criteria |
The syllabus of Biochemistry I was defined in relation to objectives and competencies to be acquired by students. In the learning modules (chapters 1-6) are discussed basic concepts about the structure, properties and functions of each of the biomolecules, so that the student acquires the ability to recognize, distinguish and describe their structure and main properties. The student must then relate and apply this knowledge to interpret and explain the different physiological functions of biomolecules from an integrated perspective.
The laboratorial component involves experimental work concerning the theoretical concepts, in which students apply their knowledge, in the execution of experimental techniques, as well as in data analysis, interpretation of results and solving problems.
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Language |
Portuguese. Tutorial support is available in English.
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