You need to activate javascript for this site.
Menu Conteúdo Rodapé
  1. Home
  2. Courses
  3. Biochemistry
  4. Analytical Methods I

Analytical Methods I

Code 12814
Year 2
Semester S1
ECTS Credits 6
Workload PL(15H)/T(30H)/TP(15H)
Scientific area Chemistry
Entry requirements Not applicable
Mode of delivery Face-to face
Work placements Not applicable
Learning outcomes Develop the students' ability to understand the theoretical knowledge underlying the analytical methods commonly used in chemical and biological laboratories and interpret the corresponding results.
At the end of this course the students should be able to:
- Know the basic principles of operation of some of the equipment most commonly used in chemical analysis laboratories and know to use it properly.
- Evaluate the benefits and limitations of different instrumental methods.
- Select an appropriate instrumental method to solve a problem in a case study, based on the theoretical knowledge and using the scientific literature as a resource.
- Interpret, explain, and accurately express analytical results.
- Be familiar with analytical methods validation procedures.
Syllabus Lectures
1 - Introduction to analytical methods
1.1 - Concepts and methods in analytical and instrumental analysis
1.2 - Statistical treatment of analytical data
1.3 – Calibration methods
1.4 – Brief introduction to analytical methods validation. Quality parameters of an analytical method.
2 - Molecular spectroscopy and photoluminescence
2.1 - Fluorescence and phosphorescence
2.2 - Instrumentation
2.3 - Quantitative applications using molecular luminescence
2.4 - Raman spectroscopy
3 - NMR spectroscopy ( two-dimensional )
4 - Mass spectrometry (application to biomolecules )
5 - Scattering spectroscopy : Turbidimetry and nephelometry
6 - Circular Dichroism

Main Bibliography - J.N. Miller, J.C. Miller,” Statistics and Chemometrics for Analytical Chemistry”, 6th Ed.,Prentice Hall, 2010.
- D.A. Skoog, F.J. Holler, S.R. Crounch, “Principles of instrumental analysis, 6th edition”, Thomson Learning,
- D.C. Harris, “Quantitative Chemical Analysis”, 7ª Ed., W. H. Freeman and Company, 2007.
- D.A. Skoog, D. West, F.J. Holler, S.R. Crounch, “Analytical Chemistry. An Introduction”, 7th Ed., Thomson
Learning, 2000
- D. Harvey, “Modern Analytical Chemistry”, 2nd Ed., McGraw-Hill, 2000.
- F.W. Fifield, D. Kealey, “Principles and Pratice of Analytical Chemistry”, 4th Ed., Chapman & Hall,1995.
Teaching Methodologies and Assessment Criteria Teaching in this curricular unit is structured into theoretical and laboratory classes. Lectures are taught with the
help of audio-visual media and give an overview of the techniques studied, with emphasis on understanding the
most important underlying concepts. Throughout the semester students have laboratory classes using
instrumental methods. There are also practical lessons where knowledge is developed through problem solving.
Language Portuguese. Tutorial support is available in English.


 [Ficheiro Local]
Lucia Silva


Last updated on: 2023-10-04

The cookies used in this website do not collect personal information that helps to identify you. By continuing you agree to the cookie policy.