You need to activate javascript for this site.
Menu Conteúdo Rodapé
  1. Home
  2. Courses
  3. Biochemistry
  4. Analytical Methods II

Analytical Methods II

Code 12819
Year 2
Semester S2
ECTS Credits 6
Workload PL(15H)/T(30H)/TP(15H)
Scientific area Chemistry
Entry requirements Not applicable
Mode of delivery Face-to-face
Work placements Not applicable.
Learning outcomes To explore the analytical methods used in analytical chemistry and biochemistry, with emphasis on chromatography and electrophoresis, electrochemical methods, methods of atomic spectroscopy and radiochemical methods as well as their equipment. The course will also explore experimental design.
At the end of this curriculum unit the student should be able to:
Explain the principles and applications of different types of electrophoresis
Explain the basic principles and mode of operation of equipment used in absorption spectroscopy / atomic
emission and select the most appropriate for solving a real case.
Explain the basic principles of operation of equipment commonly used in electrochemical methods and select the most suitable for a study case.
Explain the principles of chromatography, GC-MS and HPLC.
Explain the basic principles of radiochemical methods.
Apply experimental design to experiments.
Syllabus Lectures
1 - Chromatographic Methods
3 - Atomic Spectroscopy Methods
4 - Electroanalytical Methods
4.2- Potentiometric Methods
4.3-Voltammetric Methods
5 - Radiochemistry
6 - Experimental Design
Laboratory classes
- Qualitative determination of essential oils by GC-MS
- Determination of Metals by Atomic Absorption.
- Conductimetry: Study of the behavior of strong electrolytes and conductimetric titration.
- Potentiometric determination of iron with potassium dichromate.
Main Bibliography - J.N. Miller, J.C. Miller,” Statistics and Chemometrics for Analytical Chemistry”, 6th Ed.,Prentice Hall, 2010.
- D.A. Skoog, F.J. Holler, S.R. Crounch, “Principles of instrumental analysis, 6th edition”, Thomson Learning,
- D.C. Harris, “Quantitative Chemical Analysis”, 7ª Ed., W. H. Freeman and Company, 2007.
- D.A. Skoog, D. West, F.J. Holler, S.R. Crounch, “Analytical Chemistry. An Introduction”, 7th Ed., Thomson
Learning, 2000
- D. Harvey, “Modern Analytical Chemistry”, 2nd Ed., McGraw-Hill, 2000.
- F.W. Fifield, D. Kealey, “Principles and Pratice of Analytical Chemistry”, 4th Ed., Chapman & Hall,1995.
Teaching Methodologies and Assessment Criteria Teaching in this curricular unit is structured into theoretical and laboratory classes. Lectures are taught with the help of audio-visual media and give an overview of the techniques studied, with emphasis on understanding the most important underlying concepts. Throughout the semester students have laboratory classes using instrumental methods. There are also practical lessons where knowledge is developed through problem solving.
Language Portuguese. Tutorial support is available in English.


 [Ficheiro Local]
Lucia Silva


Last updated on: 2024-03-31

The cookies used in this website do not collect personal information that helps to identify you. By continuing you agree to the cookie policy.