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Biochemistry
Physical Biochemistry

Physical Biochemistry

Code	12832
Year	3
Semester	S2
ECTS Credits	6
Workload	PL(15H)/T(30H)/TP(15H)
Scientific area	Biochemistry
Mode of delivery	face-to-face
Work placements	Not applicable
Learning outcomes	Apply electrochemistry as analysis tool in biological systems. Operate with software available for biochemistry. Utilize the principles of data analysis and programming with free software. Apply biophysical methods in the study of biochemical systems. Describe the main applications of electrochemistry in biochemical systems. Analyze the results obtained by electrochemical techniques. Select appropriate electrochemical technique to use in a new process . Execute tasks with the use of software available to the biochemistry. Use free software (R)to create basic routines. Apply computer tools for data analysis in biochemistry. Use computer tools in the analysis and prediction of protein structures. Describe the application of hydrodynamic methods in biochemistry. Analyze calorimetric results obtained with biological systems. Describe the principles of protein crystallography.
Syllabus	1. Bioelectrochemistry 1.1. Electrochemical tools in biochemistry 1.2. Biofuel cells 1.3. Electrochemistry of redox enzymes 1.4. Electrochemical biosensors 1.5 Electrochemical immunoassays 2. Bioinformatics 2.1. Internet resources for Biochemistry 2.2. Visualization of biomolecules 2.3. Data analysis in biochemistry 2.4. Molecular modelling 2.5. Protein sequence analysis 3. Biophysical Methods 3.1. Hydrodynamic methods 3.2. Biocalorimetry 3.3. Protein crystallography Laboratory 1. Study of potentiostat’s working principle, construction of a reference electrode. 2. Cyclic voltammetry, study of redox process 3. Construction and testing of a biosensor based on PPO enzyme 4. Crystallization of a protein 5. Visualisation of biomolecules with free distribution software 6. Programming simple routines
Main Bibliography	P. N. Bartlett, “Bioelectrochemistry: Fundamentals, Experimental Techniques and Applications”, John Wiley & Sons Ltd, 2008. C. M. A. Brett, A.M.O. Brett, “Electroanalysis”, Oxford Chemistry Primers, 1998. C. S. Tsai, "An Introduction to Computational Biochemistry", Wiley-LISS, 2002. V. Bloomfield, “Computer Simulation and Data Analysis in Molecular Biology and Biophysics - An Introduction Using R”, Springer, 2009. D. Sheehan, "Physical Biochemistry: Principles and Applications", John Wiley & Sons, 2009. D. E. McRee, "Practical Protein Crystallography", Academic Press, 1999.
Language	Portuguese. Tutorial support is available in English.