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Bioprocess Design

Code 12869
Year 1
Semester S2
ECTS Credits 6
Workload OT(30H)/PL(30H)
Scientific area Bioengenharia
Entry requirements There are no prerequisites in the Bioprocess Design Curricular Unit.
Mode of delivery Tutorial system where it will be evaluated the formal exhibition of theoretical fundaments and methodologies in the response to problems. Some relevant papers to the Curricular Unit will be proposed to be studied individually. Also, will be proposed dimensioning examples in an industrial platform perspective, for potential implementation of bioreactors, chromatographic columns and other unitary methods. At this stage, creative and integrative development of students will be invoked.
Work placements Not applicable
Learning outcomes Identify and describe the fundamental principles that define bioprocesses. Understand the sustainable and global integration of a bioprocess. Understand the selection of the calculation base. Model the optimization and validation of biological systems by DOE. Identify and apply the main experimental design tools in the upstream and downstream stages of a biotechnological process.
The student must acquire the following skills:
- Knowledge of modeling in biotechnology and use them in the formulation and discussion of problems.
- Professional skills, namely: reasoning, formulation of hypotheses, systemic, creative and critical thinking, in order to handle programming in DOe.
- Know the phases of design and development of bioprocesses
Syllabus OT: 1- The classic structure of a biotechnological process: upstream, downstream and final polishing.
2- Scale-up of fermenters focusing on classic inputs (pH, temperature, culture medium, aeration and mass transfer coefficient (KLa), among others) and dimensioning to maximize the target output by applying factorial design.
3- Downstream stage scale-up: understand the main target parameters and challenges associated with Bioseparation, as well as the objectives of modeling chromatographic processes by applying experiment design.
4- Bioinformatics in the modeling, optimization and validation of the expression of bioproducts in typical biological systems. Application of factorial design, neural networks and MATLAB, in order to increase mass and volumetric productivity.
PL: Detailed study of the integration of bioprocesses: Design of penicillin production, production of chiral molecules of pharmaceutical interest; production of vitamins, among other examples.
Main Bibliography 1- Artigos científicos enquadrados nos conteúdos elaborados para a unidade curricular, nomeadamente nos domínios de modelação e validação das várias etapas de um bioprocesso.
2- Montgomery, D.C. (2001), Design and Analysis of Experiments, 5.ª ed., John Wiley & Sons, New York.
3- Guiochon G, Beaver LA, Separation science is the key to successful biopharmaceuticals, Journal of Chromatography A, 1218 (2011) 8836–8858.
4- Valente JFA, Sousa A, Queiroz JA, Sousa F, DoE to improve supercoiled p53-pDNA purification by O-phospho-L-tyrosine chromatography. Journal of Chromatography B. (2019) 1105: 184-192.
Teaching Methodologies and Assessment Criteria Teaching is student-centered, and their active participation in the learning process will allow for a greater development of their reasoning skills. The pedagogical methodology is based on face-to-face teaching and by educational objectives in problem-based learning. The teacher/tutor guides the students in the search for relevant information to obtain the expected results. The experimental modeling work will integrate all the material for the application of the acquired concepts, both in the execution of computer procedures, as in the analysis of data, interpretation of results, optimization and validation of problems. AT: two tests overall weight of 65% in the final grade. T1 and T2: with a partial weight each of 50%. Weight PL – 35% (minimum passing grade 9.5 points). The PL component encompasses the resolution of a case study proposed by the teacher at the beginning of the school year where the various groups must model, present and discuss at the end of the semester.
Language Portuguese. Tutorial support is available in English.
Last updated on: 2024-03-06

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