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Bioprocess Design

Code 12869
Year 1
Semester S2
ECTS Credits 6
Workload OT(30H)/PL(30H)
Scientific area Bioengenharia
Entry requirements There are no prerequisites in the Bioprocess Design Curricular Unit.
Mode of delivery Tutorial system where it will be evaluated the formal exhibition of theoretical fundaments and methodologies in the response to problems. Some relevant papers to the Curricular Unit will be proposed to be studied individually. Also, will be proposed dimensioning examples in an industrial platform perspective, for potential implementation of bioreactors, chromatographic columns and other unitary methods. At this stage, creative and integrative development of students will be invoked.
Work placements Not applicable
Learning outcomes Identify and describe the fundamental principles that define typical bioprocesses. Understand the sustainable and global integration of a bioprocess. Understand the selection of the calculation basis. Model
the optimization and validation of biological systems by DOE and MATLAB. Identify and apply the main tools of experimental design in the upstream stage and downstream of a biotechnological process.
Learning outcomes of the course unit:
- Knowledge about modulation in biotechnology and be able to solve problems resultant of optimization and validation.
- Personal and professional attitudes like: thought, hypothesis construction, systemic, creative and critical thinking in order to operate programming tools in MATLAB and DOE.
- To know all the steps of the design and development of bioprocesses.
Syllabus OT: 1- The classic structure of a biotechnological process: upstream, downstream and final polishing.
2- Scale-up of fermenters focusing on the classical inputs (pH, temperature, culture medium, aeration and mass transfer coefficient (KLa), among others) and dimensioning to maximize the target output applying factorial design.
3- Scale-up of the downstream step: target parameters and sizing, applying factorial design in order to increase the yield and purity of the target product.
4- Bio informatics in the modeling, optimization and validation of the expression of bioproducts in typical biological systems. Application of
factorial design, neural networks and MATLAB, in order to increase the mass and volumetric productivities.
PL: Detailed analysis of case studies integration of bioprocesses: Design of penicillin production, production of chiral molecules of harmaceutical interest; Production of vitamins, among other examples.
Main Bibliography (1) Scientific articles in the fields of modeling and validation of the various stages of a bioprocess.
(2) Doran, P.M.; “BIOPROCESS ENGINEERING PRINCIPLES”; Academic Press, 1995.
(3) Hanselman and Littlefield, Mastering MATLAB 6: “A Comprehensive Tutorial and Reference”, Prentice Hall, 2001.
(4) Montgomery, D. C., Design and Analysis of Experiments, 5.ª ed., John Wiley & Sons, New York. 2001.
(5) Pedro AQ, Martins LM, Dias JM, Bonifácio MJ, Queiroz JA, Passarinha LA. An artificial neural network for membrane-bound catechol-O-methyltransferase biosynthesis with Pichia pastoris methanol-induced cultures. Microb Cell Factories., 2015, 7; 14:113. doi: 10.1186/s12934-015-0304-7.
(6) Almeida AM, Queiroz JA, Sousa F, Sousa A. Optimization of supercoiled HPV-16 E6/E7 plasmid DNA purification with arginine monolith using design of experiments. J Chromatogr B Analyt Technol Biomed Life Sci., 2015, 26:145-50. doi: 10.1016/j.jchromb.2014.12.004.
Teaching Methodologies and Assessment Criteria OT and TP classes online via google meets and Zoom. AT Google Meets: two tests with an overall weight of 65% in the final grade. Tonline 1: with a partial weight of 50% in the theoretical final grade (7 May 2020). Tonline 2: with a partial weight of 50% in the theoretical final grade (June 2020). To pass the T component of the course, the arithmetic average of the two tests must be greater than or equal to 9.5 points. All students are admitted to the exam. A final classification equal to or higher than 9.5 is required for approval in the chair. Assessment TP - 35% (minimum passing grade 9.5). Resolution of a case study with delivery date and discussion June 2020. Online classes (OT and TP) without mandatory attendance. Notes higher or equal to 17 values, in OT, will have to be validated via oral discussion.
Language Portuguese. Tutorial support is available in English.
Last updated on: 2020-07-27

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