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Code 12870
Year 1
Semester S1
ECTS Credits 6
Workload TP(60H)
Scientific area Biotecnologia
Entry requirements Not applicable.
Mode of delivery Face-to-face.
Work placements Not applicable.
Learning outcomes The unit of Biotechnology aims to provide students with a global perspective of biotechnological processes, with emphasis on the production and purification of biomolecules for therapeutic application. At the end of this course the student should be able to: -Describe the importance of Biotechnology in the current context and explain their applications, focusing on health. -Demonstrate an integrated view of bioprocesses, including the processes of production in bioreactors, isolation and purification of biomolecules and apply the techniques / unit operations used in different stages to obtain bioproducts for therapeutic applications with high yield and purity. -Demonstrate strategy and autonomy in making decisions regarding the resolution of concrete problems in Biotechnology, based on the scientific and technical capacity and critical thinking.
Syllabus A-Theoretical 1- Introduction to Biotechnology. Recombinant DNA technology. Overexpression of recombinant proteins. Expression systems in prokaryotes and eukaryotes. 2- Biological Reactors Main state variables to be controled in a bioreactor. Fermentation general concepts . Introduction to biological systems mass balances . Growth, consumption and production kinetics. 3- Separation and Purification of Biological Products Solid-liquid. Liquid-liquid. Cell disintegration. Membrane processes. Chromatographic processes used in the purification of biological products. Overview of the steps of a biotechnological process. 4- Biotechnology applications: Gene Therapy and DNA Vaccines. B-Experimental Production and purification of human catechol-O-methyltransferase by hydrophobic interaction chromatography.
Main Bibliography 1. Required reading - Crommelin D.J. A., Sindelar, R D., Meibohm (Eds) “Pharmaceutical Biotechnology: Fundamentals and Applications”, 3rd Edition, CRC Press, 2016. - Ho R. J. Y., Gibaldi, M “Biopharmaceutical Technologies and Processes in Drug Development”, Wiley, 2003. -Lima N, Mota M. (Eds) “Biotecnologia: Fundamentos e Aplicações”. Lidel, Lisboa, 2003. - Stanbury P., Whitaker, A. Hall, S. “Principles of Fermentation Technology” 3rd Edition, Butterworth-Heinemann, 2016. -Fonseca M M, Teixeira JA (Eds) “Reactores Biológicos-Fundamentos e Aplicações”, Lidel, 2007. -Nicholl, D “An Introduction to Genetic Engineering”, 3ª Ed., Cambridge, 2008, Pg. 51-89 e pg 202-225. -Blankenstein T, “Gene Therapy: Principles and Applications”, Birkhäuser Verlag, 2003. -Selected scientific papers
Teaching Methodologies and Assessment Criteria A.1- A.1- The evaluation of Learning Modules (TP) correspondes to 65% of the final mark and includes 3 tests (30% + 16% + 19%), where it will be necessary to obtain an average minimum grade of 9.5 (Val) for approval to this component: 1st test: Mod 1: 10% (3 pt) for nf TP. Mod 2: 20% (6 pt) for nf TP. 2nd test: Mod 3: 16% (5 pt) for nf TP. 3rd Test: Mod 4: 16% (5 pt) for nf TP. Mod 5: 3% (1 pt) for nf TP A.2 -Assessment of the practice (20%): 1st test (5 pt), 2nd test (5 pt), 3rd test (5pt + 5 pt) A.3- The evaluation of the Seminar component (15%) will be done through the presentation and oral discussion of a scientific article before a jury constituted by the professors of the UC, respecting an evaluation grid previously presented to the students. It is necessary to obtain a minimum grade of 9,5 Val for approval to this component Examination (65% Mod TP + 20% Mod PL) + Seminar Note (15%)
Language Portuguese. Tutorial support is available in English.
Last updated on: 2020-01-16

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