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Biotechnology

Code 12870
Year 1
Semester S1
ECTS Credits 6
Workload TP(60H)
Scientific area Biotecnologia
Entry requirements Not applicable.
Mode of delivery Face to face.
Work placements Not applicable
Learning outcomes The unit of Biotechnology aims to provide students with a global perspective of biotechnological processes, with emphasis on the production and purification of biomolecules for therapeutic application.
At the end of this course the student should be able to:
-Describe the importance of Biotechnology in the current context and explain their applications, focusing on health.
-Demonstrate an integrated view of bioprocesses, including the processes of production in bioreactors, isolation and purification of biomolecules and apply the techniques / unit operations used in different stages to obtain bioproducts for therapeutic applications with high yield and purity.
-Demonstrate strategy and autonomy in making decisions regarding the resolution of concrete problems in Biotechnology, based on the scientific and technical capacity and critical thinking.
Syllabus A-Theoretical
1- Introduction to Biotechnology. Recombinant DNA technology. Expression systems in prokaryotes and eukaryotes.
2- Biological Reactors
Main state variables to be controled in a bioreactor. Fermentation general concepts . Introduction to biological systems mass balances. Growth, consumption and production kinetics.
3- Recovery and Separation of Biological Products
4- Separation and Purification of Biological Products
Solid-liquid. Liquid-liquid. Cell desintegration. Membrane processes. Chromatographic processes used in the purification of biological products. Overview of the steps of a biotechnological process.
5- Applications of Biotechnology: Gene Therapy and DNA Vaccines
B-Experimental
Production and purification of plasmid DNA for therapeutic applications:
1 – Plating, selection and batch production; 2 – Cell disintegration and recovery; 3 - Purification by chromatography; 4 - In vitro analysis of the gene expression.
Main Bibliography - Crommelin D.J. A., Sindelar, R D., Meibohm “Pharmaceutical Biotechnology: Fundamentals and Applications”, 3rd Edition, CRC Press, 2016.
- Ho R. J. Y., Gibaldi, M “Biopharmaceutical Technologies and Processes in Drug Development”, Wiley, 2003.
-Lima N, Mota M. (Eds) “Biotecnologia: Fundamentos e Aplicações”. Lidel, Lisboa, 2003.
- Stanbury P., Whitaker, A. Hall, S. “Principles of Fermentation Technology” 3rd Edition, Butterworth-Heinemann, 2016.
-Fonseca M M, Teixeira JA (Eds) “Reactores Biológicos-Fundamentos e Aplicações”, Lidel, 2007.
-Nicholl, D “An Introduction to Genetic Engineering”, 3ª Ed., Cambridge, 2008, Pg. 51-89 e pg 202-225.
-Blankenstein T, “Gene Therapy: Principles and Applications”, Birkhäuser Verlag, 2003.
-Selected scientific papers
Teaching Methodologies and Assessment Criteria A.1- The evaluation of TP modules corresponds to 70% of the final mark (FM) and includes 3 tests where it will be necessary to obtain an average minimum grade of 9.5 (Val) for approval to A1:
1st test: Mod 1: Mod 1:18 % + Mod 2: 24% .
2nd test: Mod 3: 24 % .
3rd Test: Mod 4: 34 % + Mod 5: 10 %

A.2 – Practical Assessment (15% of FM) in total of 4 practical works (20 pt): 1st test (5 pt), 2nd test (5 pt), 3rd test (includes 2 works: 5pt + 5 pt). Minimum grade of 9,5 pt for approval to A2.

A.3- The evaluation of the Seminar (15% of FM) includes a presentation and oral discussion of a scientific paper tried by a jury of Biotechnology professors, respecting an evaluation grid previously provided to the students. Minimum grade of 9,5 pt for approval to A3.

Frequency FM: 70% Mod TP + 15 % Mod PL + 15% Seminar
The exam corresponds to Mod TP and/or Mod PL
Exam FM: 70% Mod TP + 15 % Mod PL + 15% Seminar
Language Portuguese. Tutorial support is available in English.
Last updated on: 2024-09-20

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