This course unit gives students an overall perspective in molecular biology and its applications, with a strong practical component, in order to acquire skills in scientific research, molecular diagnosis, and forensic science.
By the end of this course the students must be able:
1–To describe the chemical and biological bases underlying the techniques of molecular biology and their practical application;
2–To describe the techniques for isolation and manipulation of nucleic acids;
3–To describe the methods for analysis of gene expression and its applications;
4–To execute the main techniques of molecular biology: DNA isolation, PCR, agarose gel electrophoresis, restriction and cloning;
5-To integrate the advanced concepts of molecular biology and to apply them to forensic sciences and to molecular diagnostic, planning experiments with critical spirit.
- Lodish H, Berk A, Zipursky SL, et al. “Molecular Cell Biology”. 4th or 5th Ed. New York: W. H. Freeman, 2000 or 2004(http://bcs.whfreeman.com/lodish5e/)
-Alberts B., Bray D., Hopkin K. et al, “Fundamentos de Biologia Celular”. 3th Ed. Artmed, 2011.
- C. Arraiano e A. Fialho, “O mundo do RNA”, 1ªEd, Lidel, 2007
- Carlos Azevedo,“Biologia Celular e Molecular”, , 4ªEd, Lidel, 2005
-“Cell Biology”,3th Ed ; A Laboratory Handbook ; Edited by: Julio E. Celis, Copyright, Elsevier Inc., 2006
- PubMed: http://www.ncbi.nlm.nih.gov/pubmed/