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Applied Molecular Biology

Code 12874
Year 1
Semester S1
ECTS Credits 6
Workload PL(30H)/T(30H)
Scientific area Bioengenharia
Mode of delivery Face-to face.
Work placements Not applicable
Learning outcomes This course unit gives students an overall perspective in molecular biology and its applications, with a strong practical component, in order to acquire skills in scientific research, molecular diagnosis, and forensic science. By the end of this course the students must be able:
1–To describe the chemical and biological bases underlying the techniques of molecular biology and their practical application;
2–To describe the techniques for isolation and manipulation of nucleic acids;
3–To describe the methods for analysis of gene expression and its applications;
4–To execute the main techniques of molecular biology: DNA isolation, PCR, agarose gel electrophoresis, restriction and cloning;
5-To integrate the advanced concepts of molecular biology and to apply them to forensic sciences and to molecular diagnostic, planning experiments with critical spirit.
Syllabus Theoretical
1.Molecular Biology: history
2.DNA, RNA and proteins
-Prokaryotic and eukaryotic genomes
-Gene expression regulation
3.Isolation of DNA and RNA
4.Manipulation of nucleic acids
-PCR and Electrophoresis
-Nucleases and modification enzymes
-Cloning vectors and recombinant molecules
-DNA sequencing
-Identification/cloning of genes from DNA libraries
5.Gene expression analysis
-RT-PCR and real time PCR
-Hybridazation, blotting and in situ Hybridazation
-Subtractive hybridization and DNA arrays
-Gel-shift and DNase I Footprinting assays
-Transfection with a reporter gene
6.Molecular biology applications
-Transgenic organisms
-Gene silence and RNAi
-Genetic markers, “DNA fingerprint” and forensic sciences
-DNA analysis and molecular diagnoses

-PCR and gel electrophoresis
-Cloning in pGEM-T
-E.coli transformation
-DNAp preparation
-DNAp restriction and analysis
-Sequencing analysis of the clone
Main Bibliography 1.Bibliografia principal
- Lodish H, Berk A, Zipursky SL, et al. “Molecular Cell Biology”. 4th or 5th Ed. New York: W. H. Freeman, 2000 or 2004(
-Alberts B., Bray D., Hopkin K. et al, “Fundamentos de Biologia Celular”. 3th Ed. Artmed, 2011.

2.Bibliografia complementar
- C. Arraiano e A. Fialho, “O mundo do RNA”, 1ªEd, Lidel, 2007
- Carlos Azevedo,“Biologia Celular e Molecular”, , 4ªEd, Lidel, 2005
-“Cell Biology”,3th Ed ; A Laboratory Handbook ; Edited by: Julio E. Celis, Copyright, Elsevier Inc., 2006
- PubMed:
Language Portuguese. Tutorial support is available in English.
Last updated on: 2016-10-06

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