Learning outcomes |
This course unit gives students an overall perspective in molecular biology and its applications, with a strong practical component, in order to acquire skills in scientific research, molecular diagnosis, and forensic science.
By the end of this course the students must be able: 1–To describe the chemical and biological bases underlying the techniques of molecular biology and their practical application; 2–To describe the techniques for isolation and manipulation of nucleic acids; 3–To describe the methods for analysis of gene expression and its applications; 4–To execute the main techniques of molecular biology: DNA isolation, PCR, agarose gel electrophoresis, restriction and cloning; 5-To integrate the advanced concepts of molecular biology and to apply them to forensic sciences and to molecular diagnostic, planning experiments with critical spirit.
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Main Bibliography |
1.Bibliografia principal - Lodish H, Berk A, Zipursky SL, et al. “Molecular Cell Biology”. 4th or 5th Ed. New York: W. H. Freeman, 2000 or 2004(http://bcs.whfreeman.com/lodish5e/) -Alberts B., Bray D., Hopkin K. et al, “Fundamentos de Biologia Celular”. 3th Ed. Artmed, 2011.
2.Bibliografia complementar - C. Arraiano e A. Fialho, “O mundo do RNA”, 1ªEd, Lidel, 2007 - Carlos Azevedo,“Biologia Celular e Molecular”, , 4ªEd, Lidel, 2005 -“Cell Biology”,3th Ed ; A Laboratory Handbook ; Edited by: Julio E. Celis, Copyright, Elsevier Inc., 2006 - PubMed: http://www.ncbi.nlm.nih.gov/pubmed/ -Blast: http://blast.ncbi.nlm.nih.gov/Blast.cgi
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