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Code 13508
Year 1
Semester S1
ECTS Credits 6
Workload TP(60H)
Scientific area Biomedical Sciences
Entry requirements Not applicable.
Mode of delivery Face to face.
Work placements Not applicable
Learning outcomes The unit of Biotechnology aims to provide students with a global perspective of biotechnological processes, with emphasis on the production and purification of biomolecules for therapeutic application.
At the end of this course the student should be able to:
-Describe the importance of Biotechnology in the current context and explain their applications, focusing on health.
-Demonstrate an integrated view of bioprocesses, including the processes of production in bioreactors, isolation and purification of biomolecules and apply the techniques / unit operations used in different stages to obtain bioproducts for therapeutic applications with high yield and purity.
-Demonstrate strategy and autonomy in making decisions regarding the resolution of concrete problems in Biotechnology, based on the scientific and technical capacity and critical thinking.
Syllabus A-Theoretical
1- Introduction to Biotechnology. Recombinant DNA technology. Expression systems in prokaryotes and eukaryotes.
2- Biological Reactors
Main state variables to be controled in a bioreactor. Fermentation general concepts . Introduction to biological systems mass balances. Growth, consumption and production kinetics.
3- Recovery and Separation of Biological Products
4- Separation and Purification of Biological Products
Solid-liquid. Liquid-liquid. Cell desintegration. Membrane processes. Chromatographic processes used in the purification of biological products. Overview of the steps of a biotechnological process.
5- Applications of Biotechnology: Gene Therapy and DNA Vaccines
Production and purification of plasmid DNA for therapeutic applications:
1 – Plating, selection and batch production; 2 – Cell disintegration and recovery; 3 - Purification by chromatography; 4 - In vitro analysis of the gene expression.
Main Bibliography - Crommelin D.J. A., Sindelar, R D., Meibohm “Pharmaceutical Biotechnology: Fundamentals and Applications”, 3rd Edition, CRC Press, 2016.
- Ho R. J. Y., Gibaldi, M “Biopharmaceutical Technologies and Processes in Drug Development”, Wiley, 2003.
-Lima N, Mota M. (Eds) “Biotecnologia: Fundamentos e Aplicações”. Lidel, Lisboa, 2003.
- Stanbury P., Whitaker, A. Hall, S. “Principles of Fermentation Technology” 3rd Edition, Butterworth-Heinemann, 2016.
-Fonseca M M, Teixeira JA (Eds) “Reactores Biológicos-Fundamentos e Aplicações”, Lidel, 2007.
-Nicholl, D “An Introduction to Genetic Engineering”, 3ª Ed., Cambridge, 2008, Pg. 51-89 e pg 202-225.
-Blankenstein T, “Gene Therapy: Principles and Applications”, Birkhäuser Verlag, 2003.
-Selected scientific papers
Teaching Methodologies and Assessment Criteria A.1- The evaluation of Learning Modules (TP) corresponds to 70% of the final mark and includes 3 tests where it will be necessary to obtain an average minimum grade of 9.5 (Val) for approval to this component:
1st test: Mod 1: Mod 1:18 % + Mod 2: 24% .
2nd test: Mod 3: 24 % .
3rd Test: Mod 4: 34 % and Mod 5: 10 %
A.2 -Assessment of the practice PL (15%): 1st test (5 pt), 2nd test (5 pt), 3rd test (5pt + 5 pt)
A.3- The evaluation of the Seminar component (15%) includes a presentation and oral discussion of a scientific paper tried by a jury of Biotechnology professors, respecting an evaluation grid previously provided to the students. It is necessary to obtain a minimum grade of 9,5 pt for approval to this component
Final Frequency Mark: 70% Mod TP + 15 % Mod PL + 15% Seminar
The exam corresponds to Mod TP or Mod PL
Final Exam mark: 70% Mod TP + 15 % Mod PL + 15% Seminar
Language Portuguese. Tutorial support is available in English.
Last updated on: 2023-10-18

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