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Applied Molecular Biology

Code 13511
Year 1
Semester S1
ECTS Credits 6
Workload TP(60H)
Scientific area Biomedical Sciences
Entry requirements Not applicable
Mode of delivery Face-to face
Work placements Not applicable
Learning outcomes This course unit gives students an overall perspective in molecular biology and its applications, with a strong practical component, in order to acquire skills in scientific research, molecular diagnosis, and forensic science.
By the end of this course the students must be able:
1–To describe the chemical and biological bases underlying the techniques of molecular biology and their practical application;
2–To describe the techniques for isolation and manipulation of nucleic acids;
3–To describe the methods for analysis of gene expression and its applications;
4–To execute the main techniques of molecular biology: DNA isolation, PCR, agarose gel electrophoresis, restriction and cloning;
5-To integrate the advanced concepts of molecular biology and to apply them to forensic sciences and to molecular diagnostic, planning experiments with critical spirit.
Syllabus THEORETICAL 1. Molecules of Molecular Biology: nucleic acids and proteins. Prokaryotic and eukaryotic genome 2. Isolation of DNA and RNA 3. Nucleic acids manipulation: Electrophoresis; PCR; Nucleases and modification enzymes; Cloning vectors and recombinant molecules; DNA sequencing; Identification of genes from banks 4. Expression of genes and proteins: RT-PCR and real-time PCR; Hybridization, blotting, In situ hybridization; Subtractive hybridizations and DNA microarrays; Next Generation RNA Sequencing, Western blot and immunocytochemistry 5. Applications of Molecular Biology: Genetic markers; DNA fingerprint and forensic sciences; Nucleic acid analysis and molecular diagnosis; Small RNAs; Genome Edition technology: CRISPR-Cas. PRACTICES (laboratory classes): Isolation of total RNA from rat liver; cDNA synthesis; PCR and electrophoresis; Cloning into a suitable vector for PCR products; E.coli transformation; Preparation of pDNA; Restriction and analysis of digestion products.
Main Bibliography 1.Required
- Lodish H, Berk A, Zipursky SL, et al. “Molecular Cell Biology”. 9th Ed. New York: W. H. Freeman, 2021
-Alberts B., Bray D., Hopkin K. et al, “Fundamentos de Biologia Celular”. 4th Ed. Artmed, 2017.

- Biología Molecular e Ingenieria Genética”, Conceptos, técnicas y aplicaciones en Ciencias de la Salud, Elsevier, 2006.
- Carlos Azevedo,“Biologia Celular e Molecular”, , 4ªEd, Lidel, 2005
-“Cell Biology”,3th Ed ; A Laboratory Handbook ; Edited by: Julio E. Celis, Copyright, Elsevier Inc., 2006
- PubMed:
Teaching Methodologies and Assessment Criteria Classes have an oral exposure of the subjects using data-show, with an intense student-teacher interaction. In order to ensure participation and motivation of the students, the debate between students and the teacher will be encouraged. Furthermore, students have the opportunity to perform the basic techniques of molecular biology, which enable them to understand all the applications. They also solve exercises related to the subjects taught.
EVALUATION: Theoretical Component (CT, 80%): Corresponds to the grade of a written global test. Practical Component (CP, 20%): Corresponding to a written report on the work developed in laboratory classes. Frequency grade = 0.8xCT+0.2xCP
FINAL EXAM: evaluation of theoretical, practical and theoretical-practical contents. Students are only admitted to the exam frequently in practical and theoretical-practical classes. Students who take the UC by exam will get the exam grade. Students who take the exam for improvement will get the highest grade.
Language Portuguese. Tutorial support is available in English.
Last updated on: 2024-01-16

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