Mode of delivery
This course unit gives students an overall perspective in molecular biology and its applications, with a strong practical component, in order to acquire skills in scientific research, molecular diagnosis, and forensic science.
By the end of this course the students must be able:
1–To describe the chemical and biological bases underlying the techniques of molecular biology and their practical application;
2–To describe the techniques for isolation and manipulation of nucleic acids;
3–To describe the methods for analysis of gene expression and its applications;
4–To execute the main techniques of molecular biology: DNA isolation, PCR, agarose gel electrophoresis, restriction and cloning;
5-To integrate the advanced concepts of molecular biology and to apply them to forensic sciences and to molecular diagnostic, planning experiments with critical spirit.
1.Molecular Biology: history
2.DNA, RNA and proteins
-Prokaryotic and eukaryotic genomes
3.Isolation of DNA and RNA
4.Manipulation of nucleic acids
-PCR and Electrophoresis
-Nucleases and modification enzymes
-Cloning vectors and recombinant molecules
-Identification/cloning of genes from DNA libraries
5.Gene expression analysis
-RT-PCR and real time PCR
-Hybridazation, blotting and in situ Hybridazation
-Subtractive hybridization and DNA arrays
-Transfection with a reporter gene
6.Molecular biology applications
-Genetic markers, “DNA fingerprint” and forensic sciences
-DNA analysis and molecular diagnoses
-Genome editing: CRISPR-Cas9
-PCR and gel electrophoresis
-Cloning in pGEM-T
-DNAp restriction and analysis
-Sequencing analysis of the clone
- Lodish H, Berk A, Zipursky SL, et al. “Molecular Cell Biology”. 6th Ed. New York: W. H. Freeman, 2008.
- Alberts B., Bray D., Hopkin K. et al, “Fundamentos de Biologia Celular”. 3th Ed. Artmed, 2011.
- Luque J., Herraez A., "Biologia Molecular e Ingenieria Genética- Conceptos, técnicas y Aplicaciones en Ciencias de la Salud". Elsevier, 2006.
- C. Arraiano e A. Fialho, “O mundo do RNA”, 1ªEd, Lidel, 2007
- Carlos Azevedo,“Biologia Celular e Molecular”, , 4ªEd, Lidel, 2005
-“Cell Biology”,3th Ed ; A Laboratory Handbook ; Edited by: Julio E. Celis, Copyright, Elsevier Inc., 2006
- PubMed: http://www.ncbi.nlm.nih.gov/pubmed/
Teaching Methodologies and Assessment Criteria
Classes have an oral exposure of the subjects using data-show, with an intense student-teacher interaction. In
order to ensure participation and motivation of the students, the debate between students and the teacher will be
encouraged.Furthermore, students have the opportunity to perform the basic techniques of molecular biology,
which enable them to understand all the applications. They also solve practical exercises related to the subjects
- Theoretical component (80% = 16 pointsj.Corresponds to the results obtained in two written tests, 8 points
each (excluding: average <6 points)
-Practical component (20% = 4 points). Corresponds to the laboratory performance (0.5 points) and to a written
report about the laboratory work (3,5 points).
Regular attendance: 70% in theoretical classes, 90% in practical classes
Portuguese. Tutorial support is available in English.