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Cell Biology: Foundations, Mechanisms and Signaling Pathways

Cell Biology: Foundations, Mechanisms and Signaling Pathways

Code	13548
Year	1
Semester	S1
ECTS Credits	6
Workload	TP(60H)
Scientific area	Biomedical Sciences
Entry requirements	N/A
Mode of delivery	Face-to-face.
Work placements	Not Applicable.
Learning outcomes	At the end of this COURSE the student should be able to: - Recognize the cell as the morphological and functional unit of living things - Know the structure and function of the main organelles of cells - Know the structure and functions of the cytoplasmic membrane - Know the structure and functions of the nucleus of the eukaryotic cell - Recognise the importance of gene expression and the regulation of this process - Meet the major metabolic pathways and cellular signaling - Recognise the importance of cell cycle regulation - Handle basic laboratory equipment - Read and understand scientific documents - Draw up an experimental protocol - Recognize its limitations and the need to maintain updated their skills, by paying special attention to the self-learning of new knowledge based on scientific evidence available - Be able to make oral presentations to different target audiences
Syllabus	Theoretical program of UC 1. The cell: The morphological and functional Unit of living things 2. The structure and function of the plasma membrane a. Transmembrane Transport 3. The components of the eukaryotic cells: a. Organelles: structure and functions b. Cytoskeleton: Composition, structure and functions 4. Cell cycle: Importance, Organization and regulation a. Mitosis b. Meiosis c. programmed cell death 5. The process of cell signaling Practical program of the UC - Basic rules of safety in the laboratory. - Obtaining, observation and manipulation of the structure of proteins and nucleic acids using a specific software (LabView Web). - Determination of ATPsintetase activity. - Isolation and culture of cells obtained from biopsies. - Sample preparation and visualization of cellular components by confocal microscopy. - Obtaining, preparation and analysis of histological sections by light microscopy.
Main Bibliography	- Fundamentos da Biologia Celular, Albert et al., 4ª edição, Artmed, 2017. - Biologia celular e molecular, Carlos Azevedo, 5ª edição, Lidel, edições técnicas, Lisboa, 2012. - Molecular Cell Biology, Lodish et al., 8th edition, W. H. Freeman and Company, 2016. - Cell and Molecular Biology: Concepts and Experiments, Karp, G. et al., 8th edition,, John Wiley & Sons, Inc., 2015.
Language	Portuguese. Tutorial support is available in English.