Biochemistry

Code	14941
Year	2
Semester	S2
ECTS Credits	6
Workload	PL(15H)/T(30H)/TP(15H)
Scientific area	Chemistry
Entry requirements	None
Learning outcomes	This Curricular Unit aims to provide students with basic knowledge in Biochemistry that will allow them to understand the molecular logic of life. At the end of this course unit the student should be able to: -Identify the general principles and terminology of Biochemistry; -Recognize and describe the structure and properties of biomolecules; -Relate the structure and properties of biomolecules with their biological function; -Identify and describe the basic principles of enzymology and enzymatic mechanisms; -Apply the knowledge acquired in the interpretation and resolution of problems in the field of Biochemistry; -Execute experimental techniques in the field of Biochemistry and analyze and interpret the results with scientific rigor and critical spirit.
Syllabus	1 INTRODUCTION TO BIOCHEMISTRY. Origin of biomolecules. The water molecule (properties and buffer solutions). 2 PROTEINS. Structure and properties of amino acids. Stereochemistry and acid-base properties. Hierarchy and structural classification of proteins. Relationship between structure and function. Allosteric proteins: hemoglobin. Protein purification and characterization. Enzyme properties. 3-GLUCOSE. General structure and properties. Stereoisomerism, mutation and forms anomeric. 4. LIPIDS. Classification, structure and properties. 5. NUCLEIC ACIDS. Structure, properties and function. 6. VITAMINS AND COENZIMES. General characteristics of water-soluble and fat-soluble vitamins. 7-PRACTICAL PROGRAM Preparation of buffer solutions. Potentiometric titration of amino acids. Determination of the concentration of a protein using the Bradford Method. Study of the kinetics of sucrose hydrolysis by the invertase enzyme.
Main Bibliography	-D.L. Nelson e M.M. Cox (2017) Lehninger Principles of Biochemistry: International Edition, 7th ed., W.H.Freeman & Co Ltd.. -J.M. Berg, J.L. Tymoczko, L. Stryer (2015) Biochemistry. 8th ed., New York: Freeman -A.Quintas, A.P Freire, M.J. Halpern, (2008) Bioquímica - Organização Molecular da Vida, Ed. Lidel -D. Voet, J.G. Voet (2011). Biochemistry. 4th ed., New York: John Wiley & Sons
Language	Portuguese. Tutorial support is available in English.