| Code |
14946
|
| Year |
3
|
| Semester |
S1
|
| ECTS Credits |
6
|
| Workload |
PL(15H)/T(30H)/TP(15H)
|
| Scientific area |
Chemistry
|
|
Entry requirements |
None
|
|
Learning outcomes |
The Instrumental Analysis Methods course aims to provide general basic knowledge of the instrumental techniques of analysis usually used in chemical analysis laboratories, namely, the UV-Vis molecular spectrophotometry, atomic absorption/emission spectroscopy, electroanalytical and chromatographic methods.
At the end of this course the students should be able to:
-know the basic principles of the equipment operation and know to use properly some of the most used in chemical analysis laboratories.
- assess the benefits and limitations of different instrumental methods.
- select an appropriate instrumental method to solve a particular problem in a case study, based on theoretical knowledge and using scientific literature as a resource.
- interpret, explain and express accurately the analytical results.
- be familiar with the analytical methods validation procedures.
|
|
Syllabus |
1-Introduction to instrumental methods of analysis
1.1- Fundamental concepts and methodologies in instrumental analysis
1.2- Calibration techniques
1.3- Basic concepts of analytical methods validation. Quality parameters of an analytical method.
2- Spectroscopic methods
2.1- UV-Vis molecular absorption spectrophotometry
2.2- Flame and electrothermal atomic absorption spectroscopy
2.3- Flame atomic emission spectroscopy and ICP
3- Chromatographic methods
3.1- Introduction
3.2- High-performance liquid chromatography
3.3- Gas chromatography
4- Electranalytical methods
4.1- Introduction to electroanalytical methods
4.2- Potentiometric methods
Laboratory assignments:
Determination of the Fe content in a food supplement by Visible absorption spectrophotometry
Determination of calcium content in drinking water by flame atomic absorption spectroscopy
Determination of fluoride content in green tea using an ESI-F
|
|
Main Bibliography |
1. D. Harris (2017). Analise Química Quantitativa, 9ª ed., LTC.
2. 1. D. A. Skoog, D. M. West, F. J. Holler, S. R. Crouch.(2013). Fundamentals of Analytical Chemistry, 9th ed., Cengage Learning, Inc., UK.
3. F. Rousseac, A. Rousseac (2007), Chemical Analysis: Modern Instrumentation Methods and Techniques, 2nd ed., Wiley.
4. G.D. Christian (2004). Analytical Chemistry.6th ed., Wiley.
5. M. L. S. Gonçalves (2001). Métodos Instrumentais para Análise de Soluções: Análise Quantitativa, 4ª ed.,
Fundação Calouste Gulbenkian.
|
|
Teaching Methodologies and Assessment Criteria |
The teaching in this unit will be structured through in-person classes, including theoretical, theoretical-practical, and laboratory sessions. In the theoretical classes, supported by audiovisual aids, a comprehensive overview of the studied techniques will be provided, with an emphasis on the fundamental concepts necessary for understanding them. In the theoretical-practical classes, knowledge will be applied through problem-solving. Throughout the semester, laboratory practices will be conducted using the instrumental methods covered.
Additionally, a seminar based on a case study will be held, focusing on the requirements for the development and application of methodologies and procedures, as well as data handling for method validation.
|
|
Language |
Portuguese. Tutorial support is available in English.
|