| Code |
15888
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| Year |
1
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| Semester |
S2
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| ECTS Credits |
5
|
| Workload |
O (92H)/PL(36H)/S(12H)
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| Scientific area |
Health Sciences
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|
Entry requirements |
Without entry requirements
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|
Learning outcomes |
Upon completion of the course, the student should:
- Have an overview of several basic and advanced techniques of fluorescence microscopy used in biomedical research.
- Have acquired fundamental notions on image analysis, such as digitizing, image enhancement, segmentation, and feature classification.
- Know how to select the image acquisition mode suitable for the intended purpose.
- Perform manual and automatic measurements using image analysis tools.
- Perform object tracking.
-Understand the applications and limitations of each technique.
- Know and apply the fundamentals of ethics in Image analysis.
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Syllabus |
Introduction to Fluorescence Microscopy
Optical Sectioning
Live Cell Imaging
Super Resolution Microscopy
Introduction to Image Analysis
Introduction to FIJI (Is Just ImageJ)
Hands-On FIJI (FRAP Analysis)
Hands-On FIJI (Colocalization Analysis)
Tips and Tricks in Fluorescence Microscopy
Introduction to Imaris
Imaris Hands-On (Tracking Analysis)
Imaris Hands-On (Morphological Analysis) Luísa Cortes
How to Analyze a large number of images: QuPath
Introduction to Huygens (Deconvolution)
Hands-On Huygens (Deconvolution)
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Main Bibliography |
Fluorescence Microscopy: From Principles to Biological Applications, 2nd Edition (2017)
Ulrich Kubitscheck (Editor) ISBN: 978-3-527-33837-5 Wiley-Blackwell
Bioimage Data Analysis, Edited by Kota Miura, Wiley-VCH
ePDF ISBN: 978-3-527-80092-6
Quantitative Imaging in Cell Biology, Methods in Cell Biology, Volume 123 (2014)
Editors Jennifer C. Waters & Torsten Wittmann
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Language |
Portuguese. Tutorial support is available in English.
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