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Instrumental Methods of Analysis

Code 15982
Year 2
Semester S2
ECTS Credits 6
Workload PL(15H)/T(30H)/TP(15H)
Scientific area Chemistry
Entry requirements -
Learning outcomes The Instrumental Analysis Methods course aims to provide general basic knowledge of the instrumental techniques of analysis usually used in chemical analysis laboratories, namely, the UV-Vis molecular spectrophotometry, atomic absorption/emission spectroscopy, electroanalytical and chromatographic methods.
At the end of this course the students should be able to:
-know the basic principles of the equipment operation and know to use properly some of the most used in chemical analysis laboratories.
- assess the benefits and limitations of different instrumental methods
- select an appropriate instrumental method to solve a particular problem in a case study, based on theoretical knowledge and using scientific literature as a resource.
- interpret, explain and express accurately the analytical results.
- be familiar with the analytical methods validation procedures.
Syllabus 1. Introduction to Instrumental Methods of Analysis.
Analytical methods classification. Introduction to statistical treatment of analytical data. Standardizing methods: linear regression and calibration curves. Brief introduction to analytical methods validation.
2. UV-Vis Absorption Spectrophotometry
Introduction. Chromophore. Beer Law. Instrumentation. Qualitative and Quantitative analysis.
3. Atomic Absorption/Emission Spectroscopy
Flame atomic absorption spectroscopy. Electrothermal atomic absorption spectroscopy. Flame atomic emission spectroscopy. Brief introduction to ICP and ICP-MS.
4. Electroanalytical Methods
Review of electrochemical basic concepts. Potentiometric Methods: Ion-selective electrodes. Voltammetric methods.
5. Chromatographic Methods
Introduction and chromatographic classification. Theoretical fundaments. Optimization of a chromatographic separation. Liquid chromatography, HPLC. Gas chromatography.
Main Bibliography D.A. Skoog, D. West, F.J. Holler, S.R. Crounch (2000). “Analytical Chemistry. An Introduction”, 7th ed., Thomson Learning.
F. Rousseac, A. Rousseac (2007), Chemical Analysis: Modern Instrumentation Methods and Techniques, 2nd ed., Wiley.
D. Harris (2008). Analise Química Quantitativa, 7ª ed., LTC.
G.D. Christian (2004). Analytical Chemistry.6th ed., Wiley.
M. L. S. Gonçalves (2001). Métodos Instrumentais para Análise de Soluções: Análise Quantitativa, 4ª ed., Fundação Calouste Gulbenkian.
Teaching Methodologies and Assessment Criteria The teaching in this course will be structured in three types of lectures theoretical, theoretical-practical and laboratory practices. In the theoretical lectures, which will be taught with the help of audiovisual equipments, will be given an overview of the technique studied, with particular focus on the most important concepts to understand them. In the theoretical-practical lectures the theoretical fundaments will be applied in problem-solving exercises. Throughout the semester will take place laboratory practices that will reinforce the material covered during lectures.
Students are asked to develop a seminar related with the development and the use of instrumental analysis methods for a given application.
The evaluation will take into account three factors of evaluation: 30% P% + 10% S + 60% T, where:
T-score of 2 partial tests and 5 mini-tests; or final exam;
S-score obtained at the seminar
P- laboratory performance evaluation, pre-lab questions and reports
Language Portuguese. Tutorial support is available in English.
Last updated on: 2024-03-02

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