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Code 9118
Year 3
Semester S2
ECTS Credits 6
Workload PL(30H)/T(30H)
Scientific area Bioengenharia
Entry requirements Not applicable
Mode of delivery Face to Face with recourse to e-learning.
Work placements Not applicable.
Learning outcomes It is intended that the student learn the fundamentals about the design, construction, operation and analysis of results obtained with biosensors. Different types of biosensors are discussed both in terms of biological recognition elements used and transduction modes.
At the end of the curricular unit (UC) the student should be able to:
1. Describe the principles of operation and signal transduction in biosensors;
2. Decide for a given biosensor on the most suitable biological element for the analyte under study, as well as the way to immobilize it;
3. Plan, design, build and evaluate the functioning of biosensors;
4. Perform measurements with biosensors;
5. Obtain and analyze the parameters that characterize the functioning of a biosensor;
6. Solve problems related to measurement equipment associated with a biosensor;
7. Work as a team to solve theoretical and practical problems.
Syllabus 1. Overview of biosensors:
1.1. Introduction to biosensor architecture.
1.2. Measurement system block diagram.
1.3. Ideal requirements.
1.4. Classification of Biosensors.
2. Molecular recognition components:
2.1. Different types of molecular recognition components - General aspects. Advantages and disadvantages.
2.2. Immobilization methods of molecular recognition components.
3. Transducers used in Biosensors.
3.1. Electrochemical Transducers:
3.2. Optical Transducers.
3.3. Miniaturization of biosensors – application of nanomaterials.
3.4. Acoustic wave transducers.
3.5. Thermal transducers.
4. Functioning parameters and evaluation of biosensors:
4.1. Validation requirements in biosensors.
4.2. Analytical Validation Characteristics of Biosensors.
5. Latest Applications of Biosensors:
5.1. Health (Point-of-care sensing).
5.2. Food Security and Water Monitoring.
5.3. Environmental Pollutants.
6. Mini-projects.
Main Bibliography • Biosensors: Fundamentals, Emerging Technologies, and Applications / edited by Edited by Sibel A. Ozkan, Bengi Uslu, and Mustafa Kemal Sezgintürk, CRC Press, Taylor & Francis Group, 2023.
• Biosensors : fundamentals and applications / edited by Bansi Dhar Malhotra and Chandra Mouli Pandey, Smithers Rapra Technology Ltd, 2017
• Chemical Sensors and Biosensors: fundamentals and Applications / edited by Florinel-Gabriel Banica, John Wiley & Sons, 2012.
• Miscellaneous articles.
• Teachers notes on the Moodle platform.
Language Portuguese. Tutorial support is available in English.
Last updated on: 2023-03-08

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