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Bioquímica I

Código 13540
Ano 2
Semestre S1
Créditos ECTS 6
Carga Horária PL(15H)/T(30H)/TP(15H)
Área Científica Bioquímica
Mode of delivery Face to Face with recourse to e-learning.
Work placements Not applicable.
Learning outcomes This Course aims to provide students with a set of knowledge in the area of basic biochemistry that enable them to understand the molecular logic of life and apply them to Biomedical Sciences.
At the end of this course the student should be able to:
-Identify the general principles and terminology of Biochemistry;
-Give examples of pathologies associated with changes in the structure and/or concentration of biomolecules;
-Recognize and describe the structure and properties of biomolecules;
-Explain the relation between the structure and properties of biomolecules and their biological function;
-Identify and describe the basic principles of enzymology and enzyme mechanisms;
-Apply the acquired knowledge to interpret and to solve problems in the fields of Biochemistry and Biomedical Sciences;
-Perform experimental techniques in the fields of Biochemistry and Biomedical Sciences and analyze and interpret the results accurately and critically.
Syllabus 1-INTRODUCTION TO BIOCHEMISTRY. Origin of biomolecules. The water molecule (properties and buffer solutions).
2-PROTEINS. Structure and properties of amino acids. Stereochemistry and acid-base properties. Hierarchy and structural classification of proteins. Structure and function of proteins. Allosteric proteins: hemoglobin. Purification and characterization of proteins. Properties of enzymes.
3-CARBOHYDRATES. Structure and general properties. Stereoisomerism, mutarotation and anomeric forms.
4-LIPIDS. Classification, structure and properties.
5-NUCLEIC ACIDS. Structure, properties and function. Plasmids.
6-VITAMINS AND COENZYMES. General features of water-soluble and fat soluble vitamins. Structure and biological role of vitamins. Coenzyme forms.
7-EXPERIMENTAL
Preparation and testing of buffer solutions.
Potentiometric titration of amino acids.
Determination of protein concentration by Bradford method.
Study of the kinetics of sucrose hydrolysis by the enzyme invertase.
Main Bibliography 1- D.L. Nelson e M.M. Cox (2017) Lehninger Principles of Biochemistry: International Edition, 7th ed., W.H.Freeman & Co Ltd, NY, USA, ISBN 1464126119

2- J.M. Berg, J.L. Tymoczko , L. Stryer (2015) Biochemistry. 8th ed., Freeman, NY, USA, ISBN 1464126100

3- A. Quintas, A.P Freire, M.J. Halpern , (2008) Bioquímica Organização Molecular da Vida, Ed. Lidel, Lisboa, Portugal, ISBN 9789727574315
Language Portuguese. Tutorial support is available in English.

Regente

Imagem d@ António Mendonça [Ficheiro Local]
 António Mendonça

Curso

Ciências Biomédicas
Data da última atualização: 2024-09-23
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